{"id":409730,"date":"2024-10-20T05:37:00","date_gmt":"2024-10-20T05:37:00","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bsi-22-30432290-dc-2022\/"},"modified":"2024-10-26T10:18:58","modified_gmt":"2024-10-26T10:18:58","slug":"bsi-22-30432290-dc-2022","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bsi-22-30432290-dc-2022\/","title":{"rendered":"BSI 22\/30432290 DC 2022"},"content":{"rendered":"

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
1<\/td>\n30432290-NC.pdf <\/td>\n<\/tr>\n
3<\/td>\nISO_DIS 22174 ed.2 – id.81777 Enquiry PDF (en).pdf <\/td>\n<\/tr>\n
7<\/td>\nForeword <\/td>\n<\/tr>\n
8<\/td>\nIntroduction <\/td>\n<\/tr>\n
9<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
15<\/td>\n4 Principle
4.1 General <\/td>\n<\/tr>\n
16<\/td>\n4.2 Test material
4.3 Sampling, transport and storage
4.4 Preparation of test sample
5 Microbial enrichment and virus concentration
5.1 Microbial enrichment
5.2 Virus concentration <\/td>\n<\/tr>\n
17<\/td>\n6 Nucleic acid preparation
6.1 General
6.2 DNA removal from dead cells
6.3 Nucleic acid extraction, release and purification
6.4 Nucleic acid quality and quantity <\/td>\n<\/tr>\n
18<\/td>\n7 PCR amplification
8 Detection and confirmation of PCR products <\/td>\n<\/tr>\n
19<\/td>\n9 General environmental laboratory requirements
9.1 General
9.2 Laboratory setup
9.2.1 General <\/td>\n<\/tr>\n
20<\/td>\n9.2.2 Control of flows <\/td>\n<\/tr>\n
21<\/td>\n9.2.3 Cleaning of laboratory
9.2.4 Environmental monitoring for PCR
10 Reagents and consumables <\/td>\n<\/tr>\n
22<\/td>\n11 Equipment
11.1 General
11.2 Specific equipment for PCR
11.2.1 Thermal cycler
11.2.2 System for detection of PCR products, comprising
11.2.3 Real-time thermal cycler
11.2.4 Digital PCR thermal cycler (for dPCR) <\/td>\n<\/tr>\n
23<\/td>\n11.2.5 Pipettes (for all PCR formats)
12 Procedure
12.1 Enrichment and sample treatment
12.2 Amplification
12.2.1 General
12.2.2 Control reaction <\/td>\n<\/tr>\n
24<\/td>\n12.2.3 Detection of amplicon
12.2.4 Data analysis
12.3 Evaluation
12.3.1 Qualitative evaluation <\/td>\n<\/tr>\n
25<\/td>\n12.3.2 Quantitative evaluation <\/td>\n<\/tr>\n
26<\/td>\n12.4 Test report
13 Performance characteristics of PCR based methods
14 Validation and verification of PCR based methods
14.1 General <\/td>\n<\/tr>\n
27<\/td>\n14.2 Validation
14.3 Verification <\/td>\n<\/tr>\n
28<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

BS EN ISO 22174. Microbiology of the food chain. Polymerase chain reaction (PCR) for the detection and quantification of microorganisms. General requirements and definitions<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2022<\/td>\n29<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":409737,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-409730","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/409730","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/409737"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=409730"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=409730"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=409730"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}