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BS ISO 10727:2002

$102.76

Tea and instant tea in solid form. Determination of caffeine content. Method using high-performance liquid chromatography

Published By Publication Date Number of Pages
BSI 2002 16
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This International Standard specifies a method for the determination by high-performance liquid chromatography (HPLC) of the caffeine content of teas and instant teas. It is applicable to green tea, black tea and decaffeinated tea products.

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PDF Pages PDF Title
3 TitlePage – Tea and instant tea in solid form�— Determination of caffeine content�— Method using …
5 Foreword – Foreword
7 Scope – 1��� Scope
NormativeReference – 2��� Normative references
Clause1 – 3��� Principle
Clause1 – 4��� Reagents
UntitledSubclause2 – 4.1��� Water
UntitledSubclause2 – 4.2��� Methanol
UntitledSubclause2 – 4.3��� Mobile phase
UntitledSubclause2 – 4.4��� Ethanol/water mixture
8 UntitledSubclause2 – 4.5��� Magnesium oxide
UntitledSubclause2 – 4.6��� Caffeine stock standard solution
UntitledSubclause2 – 4.7��� Caffeine standard solutions
UntitledSubclause3 – 4.7.1��� Caffeine standard solution�A
UntitledSubclause3 – 4.7.2��� Caffeine standard solution�B
UntitledSubclause3 – 4.7.3��� Caffeine standard solution�C
UntitledSubclause3 – 4.7.4��� Caffeine standard solution�D
Clause1 – 5��� Apparatus
UntitledSubclause2 – 5.1��� High-performance liquid chromatograph
UntitledSubclause2 – 5.2��� Chromatographic column for HPLC
9 UntitledSubclause2 – 5.3��� Filter
UntitledSubclause2 – 5.4��� Electric heating mantle
UntitledSubclause2 – 5.5��� Flask
UntitledSubclause2 – 5.6��� Anti-bumping granules
UntitledSubclause2 – 5.7��� Condenser
UntitledSubclause2 – 5.8��� Analytical balance
Clause1 – 6��� Sampling
Clause1 – 7��� Preparation of test sample
Clause1 – 8��� Procedure
Subclause2 – 8.1��� General
Subclause2 – 8.2��� Determination of dry matter content
10 Subclause2 – 8.3��� Test portion
Subclause3 – 8.3.1��� Green tea and black tea
Subclause3 – 8.3.2��� Instant tea
Subclause2 – 8.4��� Extraction of caffeine
UntitledSubclause3 – 8.4.1��� Add, to the tea in the flask,
UntitledSubclause3 – 8.4.2��� Mix the contents. Attach the condenser (
UntitledSubclause3 – 8.4.3��� Remove the flask and contents from the heating mantle and cool to r…
UntitledSubclause3 – 8.4.4��� Leave the flask to stand to allow the contents to settle, then remo…
Subclause2 – 8.5��� Dilution
Subclause2 – 8.6��� Determination
Subclause3 – 8.6.1��� Adjustment of the apparatus
Subclause3 – 8.6.2��� HPLC analysis
11 Clause1 – 9��� Calculation
Clause1 – 10��� Precision
Subclause2 – 10.1��� Interlaboratory test
12 Subclause2 – 10.2��� Repeatability
Subclause2 – 10.3��� Reproducibility
Clause1 – 11��� Test report
13 AnnexInformative – Results of interlaboratory test
14 Bibliography – Bibliography
BS ISO 10727:2002
$102.76