BS ISO 10727:2002
$102.76
Tea and instant tea in solid form. Determination of caffeine content. Method using high-performance liquid chromatography
Published By | Publication Date | Number of Pages |
BSI | 2002 | 16 |
This International Standard specifies a method for the determination by high-performance liquid chromatography (HPLC) of the caffeine content of teas and instant teas. It is applicable to green tea, black tea and decaffeinated tea products.
PDF Catalog
PDF Pages | PDF Title |
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3 | TitlePage – Tea and instant tea in solid form�— Determination of caffeine content�— Method using … |
5 | Foreword – Foreword |
7 | Scope – 1��� Scope NormativeReference – 2��� Normative references Clause1 – 3��� Principle Clause1 – 4��� Reagents UntitledSubclause2 – 4.1��� Water UntitledSubclause2 – 4.2��� Methanol UntitledSubclause2 – 4.3��� Mobile phase UntitledSubclause2 – 4.4��� Ethanol/water mixture |
8 | UntitledSubclause2 – 4.5��� Magnesium oxide UntitledSubclause2 – 4.6��� Caffeine stock standard solution UntitledSubclause2 – 4.7��� Caffeine standard solutions UntitledSubclause3 – 4.7.1��� Caffeine standard solution�A UntitledSubclause3 – 4.7.2��� Caffeine standard solution�B UntitledSubclause3 – 4.7.3��� Caffeine standard solution�C UntitledSubclause3 – 4.7.4��� Caffeine standard solution�D Clause1 – 5��� Apparatus UntitledSubclause2 – 5.1��� High-performance liquid chromatograph UntitledSubclause2 – 5.2��� Chromatographic column for HPLC |
9 | UntitledSubclause2 – 5.3��� Filter UntitledSubclause2 – 5.4��� Electric heating mantle UntitledSubclause2 – 5.5��� Flask UntitledSubclause2 – 5.6��� Anti-bumping granules UntitledSubclause2 – 5.7��� Condenser UntitledSubclause2 – 5.8��� Analytical balance Clause1 – 6��� Sampling Clause1 – 7��� Preparation of test sample Clause1 – 8��� Procedure Subclause2 – 8.1��� General Subclause2 – 8.2��� Determination of dry matter content |
10 | Subclause2 – 8.3��� Test portion Subclause3 – 8.3.1��� Green tea and black tea Subclause3 – 8.3.2��� Instant tea Subclause2 – 8.4��� Extraction of caffeine UntitledSubclause3 – 8.4.1��� Add, to the tea in the flask, UntitledSubclause3 – 8.4.2��� Mix the contents. Attach the condenser ( UntitledSubclause3 – 8.4.3��� Remove the flask and contents from the heating mantle and cool to r… UntitledSubclause3 – 8.4.4��� Leave the flask to stand to allow the contents to settle, then remo… Subclause2 – 8.5��� Dilution Subclause2 – 8.6��� Determination Subclause3 – 8.6.1��� Adjustment of the apparatus Subclause3 – 8.6.2��� HPLC analysis |
11 | Clause1 – 9��� Calculation Clause1 – 10��� Precision Subclause2 – 10.1��� Interlaboratory test |
12 | Subclause2 – 10.2��� Repeatability Subclause2 – 10.3��� Reproducibility Clause1 – 11��� Test report |
13 | AnnexInformative – Results of interlaboratory test |
14 | Bibliography – Bibliography |