BS ISO 21115:2019

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Water quality. Determination of acute toxicity of water samples and chemicals to a fish gill cell line (RTgill-W1)

Published By	Publication Date	Number of Pages
BSI	2019	48

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Categories: 13.060.70 - Examination of biological properties of water, BSI

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Description

This document specifies a method for the determination of fish acute toxicity using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. Cells in confluent monolayers in 24-well tissue culture plates are exposed to water samples, such as surface waters or different kinds of effluents, or to chemicals for 24 h and, thereafter, cell viability is assessed based on fluorescent cell viability indicator dyes (see 4.1). Data are then expressed as a percentage of unexposed control and toxicity quantified based on the percentage of cell viability versus the percentage of effluent or the chemical concentration in response curves (see Clause 9).

Additional information

Status	Under Review
Pages	48
Publication Date	2019-04-09
ISBN	978 0 580 98655 0
Standard Number	BS ISO 21115:2019
Title	Water quality. Determination of acute toxicity of water samples and chemicals to a fish gill cell line (RTgill-W1)
Identical National Standard Of	ISO 21115
Descriptors	Chemical tests, Biological tests, Water testing, Examination (education), Testing
Publisher	BSI
Committee	EH/3/5
ICS Codes	13.060.70 - Examination of biological properties of water

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BS ISO 21115:2019

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