ASTM-D7463 2008

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D7463-08 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures and Fuel Associated Water

Published By	Publication Date	Number of Pages
ASTM	2008	7

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Categories: 07.100.99 - Other standards related to microbiology, ASTM

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Description

ASTM D7463-08

Historical Standard: Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures and Fuel Associated Water

ASTM D7463

Scope

1.1 This test method provides a protocol for capturing, extracting and quantifying the adenosine triphosphate (ATP) content associated with:

1.1.1 Microorganisms found in conventional liquid fuels with kinematic viscosities (at 40°C) of ≤ 8 mm² · s^–1 as described in Table X6.1,

1.1.2 Microorganisms found in fuel-associated bottom water, and

1.1.3 Extracellular (non-cellular) ATP present in the sample matrix.

1.2 The ATP is measured using a patented bioluminescence enzyme assay, whereby light is generated in amounts proportional to the concentration of ATP in the sample. The light is produced and measured quantitatively using dedicated ATP test pens and a dedicated luminometer and reported in (instrument specific) Relative Light Units.

1.3 This test method is equally suitable for use in the laboratory or field.

1.4 Although bioluminescence is a reliable and proven method for qualifying and quantifying ATP, this method does not differentiate between ATP from different sources, for example, from different types of microorganism such as bacteria or fungi.

1.5 For water or capture solution samples, the concentration range of ATP detectable by this test method is 1 × 10^–11 M to 3 × 10^–8 M which is equivalent to 1 × 10^–14 moles/mL to 3 × 10^–11 moles/mL for water samples or capture solution. Assuming testing on fuel phase is performed on a 500 mL volume of fuel the equivalent concentrations is fuel would be: 6 × 10^–11 M to 2 × 10^–14 M.

1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Keywords

adenosine triphosphate; ATP; bacteria; biocontamination; biodeterioration; biomass; fuel; fungi; microbe; microbiology

ICS Code

ICS Number Code 07.100.99 (Other standards related to microbiology)

DOI: 10.1520/D7463-08

PDF Catalog

PDF Pages	PDF Title
1	Scope Referenced Documents Terminology
2	Summary of Test Method Significance and Use Interferences
3	Apparatus Reagents and Materials Sampling, Test Specimens, and Test Units Calibration and Standardization Procedure FIG. 1
4	FIG. 2
5	Calculation and Report Precision and Bias Keywords X1. DETERMINATION OF FUEL PHASE ATP (FUEL-WATER SAMPLES WITH 10 mL FREE-WATER) X1.1 X1.2 X1.3 X1.4
6	X2. DETERMINATION OF WATER-PHASE ATP (FUEL-WATER SAMPLES WITH 10 mL FREE-WATER) X2.1 X2.2 X2.3 X2.4 X3. DETERMINATION OF EXTRACELLULAR ATP X3.1 X3.2 X3.3 X3.4 X3.5 X3.6 X4. RESOLVING MATRIX INTERFERENCES IN WATER SAMPLES X4.1 X4.2 X4.3 X4.4 X4.5 X4.6 X5. STANDARD CURVE, METHOD X5.1 X5.2 X5.3 X5.4 X5.5 X5.6 X5.7
7	X6. TYPICAL KINEMATIC VISCOSITIES FOR VARIOUS FUELS X6.1 TABLE X6.1

Additional information

Standard Title	D7463-08 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures and Fuel Associated Water
Published Code	ASTM
Publication Date	2008
Pages Count	7
ICS Codes	07.100.99 - Other standards related to microbiology