ASTM-D4148:2012 Edition

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D4148-82(2012) Standard Test Method for Analysis of Phytoplankton in Surface Water by the Sedgwick-Rafter Method

Published By	Publication Date	Number of Pages
ASTM	2012	4

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Categories: 13.060.10 - Water of natural resources, ASTM

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Description

ASTM D4148-82-Reapproved2012

Withdrawn Standard: Standard Test Method for Analysis of Phytoplankton in Surface Water by the Sedgwick-Rafter Method (Withdrawn 2020)

ASTM D4148

Scope

1.1 This test method covers determining the density and taxonomic classification of phytoplankton. It is applicable both to relatively sparse or dense phytoplankton concentrations, provided the suspended-sediment concentration is low. The Sedgwick Rafter (S-R) method requires less costly apparatus than does the inverted microscope method but gives less accurate results. The inherent inaccuracy in the Sedgwick-Rafter method is due to the design of the counting chamber and cannot be circumvented by a different choice of optics. For this reason, the S-R method is limited to the use of objective lenses having a working distance of approximately 1.6 mm or more. With 10× oculars the maximum overall magnification is approximately 250×. High concentrations of suspended sediment can obscure the algal cells, and thus cause interference.

1.2 This test method is applicable to both freshwater and marine samples.

1.3 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific precautionary information see Section 8.

Keywords

ICS Code

ICS Number Code 13.060.10 (Water of natural resources)

DOI: 10.1520/D4148-82R12

Additional information

Standard Title	D4148-82(2012) Standard Test Method for Analysis of Phytoplankton in Surface Water by the Sedgwick-Rafter Method
Published Code	ASTM
Publication Date	2012
Pages Count	4
ICS Codes	13.060.10 - Water of natural resources