ASTM-F3088 2022(Redline)

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F3088-22 Standard Practice for Use of a Centrifugation Method to Quantify/Study Cell-Material Adhesive Interactions (Redline)

Published By	Publication Date	Number of Pages
ASTM	2022	21

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Categories: 11.100.99 - Other standards related to laboratory medicine, ASTM

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Description

ASTM F3088-22

Redline Standard: Standard Practice for Use of a Centrifugation Method to Quantify/Study Cell-Material Adhesive Interactions

ASTM F3088

Scope

1.1 This practice covers a centrifugation cell adhesion assay that can be used to detect changes in adhesive characteristics of cells with passage or treatments. This approach measures the force required to detach cells from a substrate. Adhesion, among many variables, may vary due to changes in the phenotype of the cells.

1.2 This practice does not cover methods to verify the uniformity of coating of surfaces, nor does it cover methods for characterizing surfaces.

1.3 The cells may include adult, progenitor, or stem cells from any species. The types of cells may include chondrocytes, fibroblasts, osteoblast, islet cells, or other relevant adherent cell types.

1.4 This practice does not cover methods for isolating or harvesting of cells. This practice does not cover methods to quantitate changes in gene expression, or changes in biomarker type or concentration, as identified by immunostaining. Nor does this practice cover quantitative image analysis techniques.

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Keywords

cell adhesion assay; centrifugation; chondrocytes; monolayer culture; serial passage; tissue culture-treated polystyrene;

ICS Code

ICS Number Code 11.100.99 (Other standards related to laboratory medicine)

DOI: 10.1520/F3088-22

Additional information

Standard Title	F3088-22 Standard Practice for Use of a Centrifugation Method to Quantify/Study Cell-Material Adhesive Interactions (Redline)
Published Code	ASTM
Publication Date	2022
Pages Count	21
ICS Codes	11.100.99 - Other standards related to laboratory medicine