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BS EN 13623:2020

BS EN 13623:2020

$167.15

Chemical disinfectants and antiseptics. Quantitative suspension test for the evaluation of bactericidal activity against Legionella of chemical disinfectants for aqueous systems. Test method and requirements (phase 2, step 1)

Published By Publication Date Number of Pages
BSI 2020 42
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This document specifies a test method and the minimum requirements for bactericidal activity of chemical disinfectant products intended to be used for treatment in aqueous systems against Legionella pneumophila that form a homogeneous, physically stable preparation when diluted with buffered ferrous hard water or hard water. Whenever Legionella pneumophila poses a risk to human health, this method is suitable for water used in cooling towers and water for general purposes, like spas, pools, showers and other uses. The method is not suitable for electro-chemical disinfection.

The document applies to products used as a single application shock treatment in order to kill Legionella pneumophila. It is not suitable for the evaluation of those products that are dosed continuously into water systems to control the growth of Legionella pneumophila.

NOTE 1

The method described is intended to determine the activity of commercial formulations or active substances under the conditions in which they are used.

NOTE 2

This method corresponds to a phase 2 step 1 test.

NOTE 3

This method does not take into account the fact that Legionella pneumophila is often found in cells of amoebae and/or biofilms and that thereby a product’s activity against the bacteria may be reduced.

EN 14885 specifies in detail the relationship of the various tests to one another and to “use recommendation”.

PDF Catalog

PDF Pages PDF Title
2 undefined
7 1 Scope
2 Normative references
3 Terms and definitions
8 4 Requirements
5 Test methods
5.1 Principle
9 5.2 Materials and reagents
5.2.1 Test organism
5.2.2 Culture media and reagents
5.2.2.1 General
5.2.2.2 Water
10 5.2.2.3 Buffered Charcoal Yeast Extract (BCYE) Agar
11 5.2.2.4 BCYE Broth
5.2.2.5 Neutralizer
5.2.2.6 Rinsing liquid (for membrane filtration)
5.2.2.7 Hard water for general purposes (HWGP)
12 5.2.2.8 Interfering substance (yeast extract)
5.2.2.9 Page’s Saline
5.2.2.10 Buffered ferrous hard water for treatment of cooling water (BFHW)
13 5.3 Apparatus and glassware
5.3.1 General
5.3.2 Usual microbiological laboratory equipment)
14 5.4 Preparation of test organism suspensions and test solutions
5.4.1 Test organism suspension (test suspension N and validation suspension NV)
5.4.1.1 General
5.4.1.2 Preservation and stock cultures of test organism
15 5.4.1.3 Working cultures of test organism
5.4.1.4 Test suspensions (N)
5.4.1.5 Validation suspension (NV, NVB)
5.4.1.6 Incubation and counting of the test and validation suspensions
16 5.4.2 Product test solution
5.5 Procedure for assessing the bactericidal activity of the product
5.5.1 General
5.5.1.1 Experimental conditions (obligatory and additional)
17 5.5.1.2 Choice of test method
5.5.1.3 General instructions for validation and control procedures
5.5.1.4 Equilibration of temperature
5.5.1.5 Precautions for manipulation of test organisms
18 5.5.2 Dilution-neutralization method)
5.5.2.1 General
5.5.2.2 Test Na – determination of bactericidal concentrations
5.5.2.3 Experimental conditions control A – validation of the selected experimental conditions and/or verification of the absence of any lethal effect in the test conditions
19 5.5.2.4 Neutralizer control B – verification of the absence of toxicity of the neutralizer
5.5.2.5 Method validation C – dilution-neutralization validation
5.5.2.6 Incubation and counting of the test mixture and the control and validation mixtures
20 5.5.3 Membrane filtration method)
5.5.3.1 General
5.5.3.2 Test Na– determination of the bactericidal concentrations
5.5.3.3 Experimental conditions control A – validation of the selected experimental conditions and/or verification of the absence of any lethal effect in the test conditions
21 5.5.3.4 Filtration control B – validation of the filtration procedure
5.5.3.5 Method validation C – validation of the membrane filtration method or counting of the bacteria on the membranes which have previously been in contact with the mixture of product and interfering substance
5.5.3.6 Incubation and counting of test mixture and the control and the validation mixtures
5.6 Experimental data and calculations
5.6.1 Explanation of terms and abbreviations
5.6.1.1 Overview of the different suspensions and test mixtures
22 5.6.1.2 VC-values
5.6.2 Calculation
5.6.2.1 General
23 5.6.2.2 Determination of VC-values
24 5.6.2.3 Calculation of N and N0
5.6.2.4 Calculation of Na
26 5.6.2.5 Calculation of NV and NV0
5.6.2.6 Calculation of A, B and C
27 5.7 Verification of methodology
5.7.1 General
5.7.2 Control of weighted mean counts
5.7.3 Basic limits
5.8 Expression of results and precision
5.8.1 Reduction
28 5.8.2 Control of active and non-active product test solution (5.4.2)
5.8.3 Bactericidal concentration
5.8.4 Precision, repetition
5.9 Interpretation of results – conclusion
5.9.1 General
5.9.2 Bactericidal activity for general purposes
5.9.3 Bactericidal activity for specific purposes
5.10 Test report
30 Annex A (informative)Referenced strains in national collections
31 Annex B (informative)Determination of the bactericidal activity against Legionella pneumophila
34 Annex C (informative)Neutralizer
36 Annex D (informative)Graphical representation of test procedures
D.1 Dilution-neutralization method
38 D.2 Membrane filtration method

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BS EN 13623:2020
$167.15