BS EN 13697:2015+A1:2019
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Chemical disinfectants and antiseptics, Quantitative non-porous surface test for the evaluation of bactericidal and/or fungicidal activity of chemical disinfectants used in food, industrial, domestic and institutional areas. Test method and requirements without mechanical action (phase 2, step 2)
Published By | Publication Date | Number of Pages |
BSI | 2019 | 42 |
This European Standard specifies a test method (phase 2/step 2) and the minimum requirements for bactericidal and/or fungicidal or yeasticidal activity of chemical disinfectants that form a homogeneous physically stable preparation in hard water or – in the case of ready-to-use products – with water in food, industrial, domestic and institutional areas, excluding areas and situations where disinfection is medically indicated and excluding products used on living tissues. The scope of this European Standard applies at least to the following: a) Processing, distribution and retailing of: 1) Food of animal origin: i) milk and milk products; ii) meat and meat products; iii) fish, seafood and products; iv) eggs and egg products; v) animal feeds; vi) etc. 2) Food of vegetable origin: i) beverages; ii) fruits, vegetables and derivatives (including sugar distillery); iii) flour, milling and backing; iv) animal feeds; v) etc. b) Institutional and domestic areas: 1) catering establishments; 2) public areas; 3) public transports; 4) schools; 5) nurseries; 6) shops; 7) sports rooms; 8) waste container (bins); 9) hotels; 10) dwellings; 11) clinically non sensitive areas of hospitals; 12) offices; 13) etc. c) Other industrial areas: 1) packaging material; 2) biotechnology (yeast, proteins, enzymes…); 3) pharmaceutical; 4) cosmetics and toiletries; 5) textiles; 6) space industry, computer industry; 7) etc. Using this European Standard, it is possible to determine the bactericidal or fungicidal or yeasticidal activity of the undiluted product. As three concentrations are tested, in the active to non active range, dilution of the product is required and, therefore, the product forms a homogeneous stable preparation in hard water. EN 14885 specifies in detail the relationship of the various tests to one another and to use recommendations. NOTE 1 The method described is intended to determine the activity of commercial formulations or active substances on bacteria and/or fungi in the conditions in which they are used. NOTE 2 This method cannot be used to evaluate the activity of products against mycobacteria.
PDF Catalog
PDF Pages | PDF Title |
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2 | National foreword |
8 | 1 Scope |
9 | 2 Normative references |
10 | 3 Terms and definitions 4 Requirements |
11 | 5 Test methods 5.1 Principle |
12 | 5.2 Materials and reagents 5.2.1 Test organisms 5.2.2 Culture media and reagents 5.2.2.1 General 5.2.2.2 Water |
13 | 5.2.2.3 Tryptone Soya Agar (TSA) 5.2.2.4 Malt extract agar (MEA) |
14 | 5.2.2.5 Diluent 5.2.2.6 Neutralizer 5.2.2.7 Hard water for dilution of the products 5.2.2.8 Interfering substances |
15 | 5.2.3 Test surface |
16 | 5.3 Apparatus and glassware |
17 | 5.4 Preparation of test organism suspensions and product test solutions 5.4.1 Test organism suspensions 5.4.1.1 Stock cultures of test organisms 5.4.1.2 Working culture of test organisms |
18 | 5.4.1.3 Test suspensions |
20 | 5.4.1.4 Counting of bacterial and fungal test suspensions |
21 | 5.4.1.5 Counting the weighed mean of the test suspensions 5.4.2 Product test solutions 5.5 Procedure 5.5.1 Choice of experimental conditions |
22 | 5.5.2 Test procedure 5.5.2.1 Test “Nd” – determination of microbicidal concentrations |
25 | 5.5.2.2 Water control “Nc” 5.5.2.3 Neutralizer control “NC” – verification of the absence of toxicity of the neutralizer |
26 | 5.5.2.4 Method validation “NT” – dilution-neutralization validation 5.5.3 Counting of the test mixtures |
27 | 5.6 Calculation and expression of results 5.6.1 Elaboration of data: counting of weighed average values |
28 | 5.6.2 Verification of methodology |
29 | 5.6.3 Expression of results 5.6.3.1 Reduction 5.6.4 Conclusion 5.6.4.1 Activity on non-porous surfaces for general purposes 5.6.4.2 Activity on non-porous surfaces for specific purposes |
30 | 5.7 Test report |
32 | Annex A (informative)Corresponding referenced strains |
34 | Annex B (informative)Neutralizers |
36 | Annex C (informative)Expression of results with the dilution-neutralization method |
38 | Annex D (informative)Bactericidal activity in surfaces in general use conditions (for clean conditions) |
40 | Annex E (informative)Precision of the test result |