BS EN 13697:2023

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Chemical disinfectants and antiseptics. Quantitative non-porous surface test for the evaluation of bactericidal and yeasticidal and/or fungicidal activity of chemical disinfectants used in food, industrial, domestic and institutional areas without mechanical action. Test method and requirements without mechanical action (phase 2, step 2)

Published By	Publication Date	Number of Pages
BSI	2023	44

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Categories: 71.100.35 - Chemicals for industrial and domestic disinfection purposes, BSI

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Description

This document specifies a test method (phase 2/step 2) and the minimum requirements for bactericidal and yeasticidal and/or fungicidal activity of chemical disinfectants that form a homogeneous physically stable preparation in hard water or – in the case of ready-to-use products – with water in food, industrial, domestic and institutional areas, excluding areas and situations where disinfection is medically indicated and excluding products used on living tissues. This document applies to products that are used for disinfecting non-porous surfaces without mechanical action. This document is applicable at least to the following: a) processing, distribution and retailing of: 1) food of animal origin: i) milk and milk products; ii) meat and meat products; iii) fish, seafood and products; iv) eggs and egg products; v) animal feeds; vi) etc. 2) food of vegetable origin: i) beverages; ii) fruits, vegetables and derivatives (including distilleries and sugar refineries); iii) flour, milling and baking; iv) animal feeds; v) etc. b) institutional and domestic areas: 1) catering establishments; 2) public areas; 3) public transports; 4) schools; 5) nurseries; 6) shops; 7) sports rooms; 8) waste containers (bins); 9) hotels; 10) dwellings; 11) clinically non sensitive areas of hospitals; 12) offices; 13) etc. c) other industrial areas: 1) packaging material; 2) biotechnology (yeast, proteins, enzymes…); 3) pharmaceutical; 4) cosmetics and toiletries; 5) textiles; 6) space industry, computer industry; 7) etc. This document does not apply when the product is applied via an automatic airborne disinfection method; in such cases, see EN 17272. Using this document, it is possible to determine the bactericidal and/or yeasticidal and/or fungicidal activity of the undiluted product. As three concentrations are tested, in the active to non-active range, dilution of the product is required and, therefore, the product forms a homogeneous stable preparation in hard water. EN 14885 specifies in detail the relationship of the various tests to one another and to use recommendations. NOTE 1 The method described is intended to determine the activity of commercial formulations or active substances on bacteria and/or fungi in the conditions in which they are used. NOTE 2 This method cannot be used to evaluate the activity of products against mycobacteria.

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PDF Pages	PDF Title
2	undefined
8	1 Scope
9	2 Normative references
10	3 Terms and definitions 4 Requirements
12	5 Test methods 5.1 Principle 5.2 Materials and reagents 5.2.1 Test organisms
13	5.2.2 Culture media and reagents 5.2.2.1 General 5.2.2.2 Water 5.2.2.3 Tryptone Soya Agar (TSA) 5.2.2.4 Malt extract agar (MEA)
14	5.2.2.5 Diluent 5.2.2.6 Neutralizer 5.2.2.7 Hard water for dilution of the products
15	5.2.2.8 Interfering substances
16	5.2.3 Test surface 5.3 Apparatus and glassware
17	5.4 Preparation of test organism suspensions and product test solutions 5.4.1 Test organism suspensions 5.4.1.1 Stock cultures of test organisms
18	5.4.1.2 Working culture of test organisms 5.4.1.3 Test suspensions
20	5.4.1.4 Quantifying of bacterial and fungal test suspensions 5.4.1.5 Calculating the weighed mean of the test suspensions
21	5.4.2 Product test solutions 5.5 Procedure 5.5.1 Choice of experimental conditions
22	5.5.2 Test procedure 5.5.2.1 Test “Na” – determination of microbicidal concentrations
25	5.5.2.2 Water control “A” 5.5.2.3 Neutralizer control “B” – verification of the absence of toxicity of the neutralizer
26	5.5.2.4 Method validation “C” – dilution-neutralization validation 5.5.3 Quantifying of the test mixtures
27	5.6 Calculation and expression of results 5.6.1 Elaboration of data: counting of weighed average values
28	5.6.2 Verification of methodology 5.6.3 Expression of results 5.6.4 Conclusion 5.6.4.1 Activity on non-porous surfaces for general purposes
29	5.6.4.2 Activity on non-porous surfaces for dairies 5.6.4.3 Activity on non-porous surfaces for specific purposes 5.7 Test report
32	Annex A (informative)Corresponding referenced strains
34	Annex B (informative)Neutralizers
36	Annex C (informative)Expression of results with the dilution-neutralization method
38	Annex D (informative)Bactericidal activity in surfaces in general use conditions (for clean conditions)
40	Annex E (informative)Precision of the test result
41	Annex F (informative)Alternative drying end point

Additional information

Status	Definitive
Pages	44
Publication Date	2023-11-07
ISBN	978 0 539 22623 2
Standard Number	BS EN 13697:2023
Title	Chemical disinfectants and antiseptics. Quantitative non-porous surface test for the evaluation of bactericidal and yeasticidal and/or fungicidal activity of chemical disinfectants used in food, industrial, domestic and institutional areas without mechanical action. Test method and requirements without mechanical action (phase 2, step 2)
Identical National Standard Of	EN 13697
Replaces	BS EN 13697:2015+A1:2019
Descriptors	Eumycophyta, Food manufacturing processes, Antiseptics, Count methods (microbiology), Biological analysis and testing, Surfaces, Disinfectants, Industrial facilities, Bacteriocide-activity determination, Food products, Microbiological analysis, Domestic facilities, Bacteria count methods, Institutional facilities, Bacteria
Publisher	BSI
Committee	CH/216
ICS Codes	71.100.35 - Chemicals for industrial and domestic disinfection purposes

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BS EN 13697:2023

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