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BS EN 14164:2014

BS EN 14164:2014

$102.76

Foodstuffs. Determination of vitamin B6 by high performance chromatography

Published By Publication Date Number of Pages
BSI 2014 20
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This European Standard specifies a method for the determination of vitamin B6 in foodstuffs by high performance liquid chromatography (HPLC). Vitamin B6 is the mass fraction of the sum of pyridoxine, pyridoxal, pyridoxamine including their phosphorylated derivatives determined as pyridoxine. The β-glycosylated forms are not taken into account. These can be determined with the method given in EN 14663 [1] by which the different vitamins of vitamin B6 (pyridoxal, pyridoxamine and pyridoxine) are separated and individually quantified. A third European Standard, EN 14166 [2], determines the total vitamin B6 by microbiological assay.

PDF Catalog

PDF Pages PDF Title
4 Contents Page
5 Foreword
6 1 Scope
2 Normative references
3 Principle
4 Reagents
10 5 Apparatus
6 Procedure
6.1 Preparation of the test sample
6.2 Preparation of the sample test solution
6.2.1 Extraction
11 6.2.2 Enzyme treatment and transformation steps
6.2.3 Identification
6.2.4 Determination by HPLC
7 Calculation
12 8 Test report
13 Annex A (informative) Example of a chromatogram
Figure A.1 — Example for an HPLC quantification of pyridoxine in milk powder
14 Annex B (informative) Precision data
Table B.1 — Precision data for the determination of vitamin B6
15 Table B.2 — Precision data for the determination of vitamin B6 in reconstituted infant formula
16 Annex C (informative) Sample treatment option without acid hydrolysis
17 Annex D (informative) Examples for molar absorption coefficients
Table D.1 — Examples for molar absorption coefficients (ε) of vitamin B6 compounds [6], [13]
18 Bibliography

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BS EN 14164:2014
$102.76