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BS EN 15633-1:2019

BS EN 15633-1:2019

$102.76

Foodstuffs. Detection of food allergens by immunological methods – General considerations

Published By Publication Date Number of Pages
BSI 2019 18
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This document provides an overall framework covering qualitative and quantitative methods for the determination of food allergens and allergenic ingredients using antibody-based methods in foods. This document specifies general guidelines and performance criteria for antibody-based methods for the detection and quantification of proteins that serve as markers for the presence of allergy provoking foods or food ingredients. Other methods than those described can also detect and identify the proteins. Guidelines, minimum requirements and performance criteria laid down in this document are intended to ensure that reproducible results are obtained by different analysts in private and/or official control laboratories or when conducting onsite food testing.

This document is intended to be used in addition to EN 15842.

NOTE

This document could also be applicable to other sample types where the same principles for method validation and verification would apply.

PDF Catalog

PDF Pages PDF Title
2 National foreword
5 European foreword
6 Introduction
7 1 Scope
2 Normative references
3 Terms and definitions
3.1 General terms
3.2 Antibody-related terms
9 3.3 Method-related terms
4 General requirements for laboratories
4.1 Principle
4.2 Apparatus and equipment
4.3 Material and reagents
10 5 Procedure
5.1 General
5.2 Sample preparation
5.3 Extraction
11 5.4 Detection
5.4.1 General
5.4.2 ELISAs
5.4.3 Lateral flow devices (LFD)/Flow through tests
5.5 Assay procedure
6 Interpretation and expression of results
6.1 General remarks
12 6.2 Quantitative results
13 6.3 Qualitative results
7 Specific parameters which can influence results
7.1 General
14 7.2 Limit of quantitation
7.3 Matrices
15 7.4 Extraction efficiency
7.5 Standard curves
7.6 Conversion factor and units
7.7 Cross-reactivity
8 Test report
16 Bibliography

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BS EN 15633-1:2019
$102.76