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BS EN 15634-4:2023 – TC

BS EN 15634-4:2023 – TC

$134.27

Tracked Changes. Foodstuffs. Detection of food allergens by molecular biological methods – Peanut (Arachis hypogaea). Qualitative detection of a specific DNA sequence in chocolate by real-time PCR

Published By Publication Date Number of Pages
BSI 2023 46
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This document specifies a method for the detection of peanut (Arachis hypogaea) in chocolate. Real-time PCR (Polymerase Chain Reaction) detection of peanut is based on an 86 bp (base pair) sequence from the Ara h 2 gene of peanut.

PDF Catalog

PDF Pages PDF Title
28 undefined
33 1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
5.1 General
5.2 Extraction reagents
34 5.3 DNA purification by means of solid phase extraction
5.4 Real-time PCR reagents
35 6 Apparatus and equipment
6.1 General
6.2 DNA extraction
6.3 PCR
36 7 Procedure
7.1 General
7.2 Sample preparation
7.3 Preparation of extracts
7.3.1 DNA extraction with CTAB and DNA purification
37 7.3.2 Quantification of DNA concentration and purity/quality check
7.4 Real-time PCR set-up
7.4.1 Reaction mix for real-time PCR
38 7.4.2 Positive control for DNA targets
7.4.3 Negative control for DNA targets
39 7.4.4 PCR inhibition control
7.4.5 Amplification reagent control
7.4.6 Extraction blank control
7.4.7 Positive extraction control
7.4.8 Temperature/time programme (real-time PCR)
7.4.9 Accept/Reject criteria
40 7.4.10 Identification
8 Validation
8.1 General
8.2 Specificity
8.3 Ring trial validation study
8.3.1 Setup of the ring trial study
41 8.3.2 Ring trial validation results
8.3.2.1 Ring trial validation data
42 8.3.2.2 Robustness of the method
8.3.2.3 Limit of detection
9 Test report

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BS EN 15634-4:2023 - TC
$134.27