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BS EN 17422:2022

$142.49

Chemical disinfectants and antiseptics. Quantitative surface test for the evaluation of teat disinfectants used in the veterinary area. Test method and requirements (phase 2 step 2)

Published By Publication Date Number of Pages
BSI 2022 30
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PDF Pages PDF Title
2 undefined
7 1 Scope
2 Normative references
3 Terms and definitions
4 Requirements
8 5 Test method
5.1 Principle
5.2 Materials and reagents
5.2.1 Test organisms
9 5.2.2 Culture media and reagents
5.2.2.1 General
5.2.2.2 Water
5.2.2.3 Tryptone soya agar (TSA)
5.2.2.4 Diluent
5.2.2.5 Neutralizer
10 5.2.2.6 Standardized hard water
5.2.2.7 Interfering substances
11 5.2.3 Test surface – synthetic skin
5.2.3.1 VITRO-SKIN®
5.3 Apparatus and glassware
5.3.1 General
5.3.2 Usual microbiological equipment,and in particular, the following:
12 5.4 Preparation of test organism suspension and product test solutions
5.4.1 Test organism suspension (test and validation suspension)
5.4.1.1 General
5.4.1.2 Preservation and stock cultures of test organisms
5.4.1.3 Working culture of test organisms
13 5.4.1.4 Test suspension (“N”)
5.4.1.5 Incubation and counting of the test suspension
5.4.2 Product test solution
14 5.5 Procedure for assessing the bactericidal activity of the product
5.5.1 General
5.5.1.1 Experimental conditions
5.5.1.2 Neutralization
5.5.1.3 General instructions for the validation and control procedures
15 5.5.1.4 Equilibration of temperature
5.5.2 Test procedure
5.5.2.1 General
5.5.2.2 Test “Na” determination of bactericidal concentrations
16 5.5.2.3 Water control “Nw”
5.5.2.4 Neutralizer control “B” – verification of the absence of toxicity of the neutralizer
5.5.2.5 Method validation “C”
17 5.6 Experimental data and calculation
5.6.1 Explanation of terms and abbreviations
5.6.1.1 Overview of the different suspensions and test mixtures
5.6.1.2 Vc values
5.6.2 Calculation
5.6.2.1 General
5.6.2.2 Determination of Vc values
18 5.6.2.3 Calculation of N
5.6.2.4 Calculation of Na and Nw
19 5.6.2.5 Calculation of B and C
20 5.7 Verification of methodology
5.7.1 General
5.7.2 Control of weighted mean “WM”counts
5.7.3 Basic limits
5.8 Expression of results and precision
5.8.1 Reduction
21 5.8.2 Control of active and non-active product test solution (5.4.2)
5.8.3 Limiting test organism and bactericidal concentration
5.8.4 Precision, repetitions
5.9 Interpretation of results – conclusion
5.9.1 General
5.9.2 Bactericidal activity for teat disinfection
22 5.10 Test report
24 Annex A (informative)Referenced strains in national collections
25 Annex B (informative)Examples of neutralizers of the residual antimicrobial activity of chemical disinfectants and antiseptics and rinsing liquids
26 Annex C (informative)Example of a typical test report
BS EN 17422:2022
$142.49