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BS EN ISO 10273:2017

BS EN ISO 10273:2017

$198.66

Microbiology of the food chain. Horizontal method for the detection of pathogenic Yersinia enterocolitica

Published By Publication Date Number of Pages
BSI 2017 52
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This document specifies a horizontal method for the detection of Y. enterocolitica associated with human disease. It is applicable to

  • products intended for human consumption and the feeding of animals, and

  • environmental samples in the area of food production and food handling.

PDF Catalog

PDF Pages PDF Title
2 National foreword
4 European foreword
7 Foreword
9 Introduction
11 1 Scope
2 Normative references
3 Terms and definitions
12 4 Abbreviated terms
5 Principle
5.1 General
5.2 Direct plating from liquid enrichment medium
5.3 Enrichment in liquid enrichment medium and selective liquid enrichment medium
5.4 Plating out after enrichment and identification
13 5.5 Confirmation
6 Culture media and reagents
7 Equipment and consumables
7.8 Peristaltic blender.
8 Sampling
14 9 Preparation of test sample
10 Procedure (as shown in Annex A)
10.1 Test portion and initial suspension
10.2 Direct plating on selective agar
15 10.3 Enrichment
10.4 Plating out and incubation of plates
10.4.1 Plating from PSB and ITC by KOH treatment on CIN agar
10.4.2 Plating from PSB and ITC by KOH treatment on chromogenic agar (optional)
10.5 Identification of characteristic colonies
16 10.6 Confirmation
10.6.1 General
10.6.2 Selection of colonies for confirmation
10.6.3 Determination of pathogenic Yersinia species
18 10.6.4 Confirmation of pathogenic Y. enterocolitica
20 10.6.5 Interpretation of confirmation tests for Y. enterocolitica
10.6.6 Interpretation of confirmation tests for pathogenic Y. enterocolitica
10.7 Biotyping of Y. enterocolitica (optional)
10.7.1 General
21 10.7.2 Fermentation of xylose
10.7.3 Tween-esterase test
10.7.4 Fermentation of salicin (optional) and trehalose
10.7.5 Indole formation
10.7.6 Interpretation of biotyping tests
22 11 Expression of results
12 Performance characteristics of the method
12.1 Interlaboratory study
12.2 Sensitivity
12.3 Specificity
12.4 LOD50
23 13 Test report
14 Quality assurance
24 Annex A (normative) Diagrams of the procedures
27 Annex B (normative) Composition and preparation of culture media and reagents
42 Annex C (informative) Method validation studies and performance characteristics
44 Annex D (informative) Procedure for cold enrichment
49 Bibliography

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BS EN ISO 10273:2017
$198.66