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BS EN ISO 16654:2001+A1:2017

BS EN ISO 16654:2001+A1:2017

$142.49

Microbiology of food and animal feeding stuffs. Horizontal method for the detection of Escherichia coli O157

Published By Publication Date Number of Pages
BSI 2017 28
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This International Standard specifies a horizontal method for the detection of Escherichia coli serogroup O157.

Subject to the limitations discussed in the introduction, this International Standard is applicable to products intended for human consumption or for animal feeding stuffs.

PDF Catalog

PDF Pages PDF Title
2 National foreword
4 European Foreword
Anchor 3
European Foreword to amendment A1
5 Annex ZA (normative) Normative references to international publications with their relevant European publications
8 Foreword
9 Introduction
11 1 Scope
2 Normative references
3 Term and definition
12 4 Principle
5 Culture media, reagents and antisera
5.1 Enrichment medium: Modified tryptone soya broth with novobiocin (mTSB + N)
5.1.1 Modified tryptone soya broth (mTSB)
5.1.2 Novobiocin solution
13 5.2 First selective isolation medium: Cefixime tellurite sorbitol MacConkey agar (CT-SMAC)
5.2.1 Base medium
5.2.2 Potassium tellurite solution
14 5.2.3 Cefixime solution
5.2.4 Complete medium
5.3 Second selective isolation medium
15 5.4 Nutrient agar
5.4.1 Composition
5.4.2 Preparation
5.4.3 Preparation of nutrient agar plates
5.5 Tryptone/tryptophan medium
5.5.1 Composition
5.5.2 Preparation
16 5.6 Kovac’s indole reagent
5.6.1 Composition
5.6.2 Preparation
5.7 Anti-Escherichia coli O157 immunomagnetic particles
5.8 Wash buffer: Modified phosphate buffer, 0,01mol/l, of pH 7,2
5.8.1 Composition
5.8.2 Preparation
5.9 Normal saline solution
5.9.1 Composition
17 5.9.2 Preparation
6 Apparatus and glassware
6.1 Apparatus for dry sterilization (oven) and/or wet sterilization (autoclave)
18 6.16 Vortex mixer
7 Sampling
8 Preparation of test sample
9 Procedure (see annex A)
9.1 Test portion and initial suspension
9.2 Enrichment
19 9.3 Immunomagnetic separation (IMS)
9.3.1 General
9.3.2 Immunocapture
9.3.3 Separation
9.4 Plating out onto selective agars and identification of E. coli O157 colonies
9.4.1 Plating out
20 9.4.2 Recognition of typical E. coli O157 colonies
9.5 Confirmation
9.5.1 Selection of colonies
9.5.2 Biochemical confirmation: Indole formation
9.5.3 Serological identification
21 9.6 Further characterization
10 Quality assurance
10.1 Test strains for quality assurance purposes
10.2 Culture method
11 Expression of results
12 Performance characteristics of the method
12.1 Interlaboratory study
12.2 Sensitivity
22 12.3 Specificity
12.4 LOD50
13 Test report
23 Annex A (normative) Diagram of procedure
24 Annex B (informative) Results of interlaboratory studies
27 Bibliography

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BS EN ISO 16654:2001+A1:2017
$142.49