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BS EN ISO 18743:2015

BS EN ISO 18743:2015

$142.49

Microbiology of the food chain. Detection of Trichinella larvae in meat by artificial digestion method

Published By Publication Date Number of Pages
BSI 2015 26
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This International Standard specifies a method of detection of Trichinella spp. muscle stage larvae in meat of individual animal carcasses intended for human consumption. It is applicable to the examination of meat from domestic and sylvatic animal species, which can be infected by nematodes of the genus Trichinella.

This method does not allow the identification of the species or genotype of detected parasites; species or genotype identification can be carried out by molecular methods.

The method described in this International Standard is intended to be used in conjunction with the guidelines in the OIE Manual of Diagnostic Tests and Vaccines and by the International Commission on Trichinellosis (ICT) for Trichinella testing and the inspection of carcasses intended for human consumption, unless it has been demonstrated by other means that the animal was not at risk for exposure to Trichinella.

The artificial digestion/magnetic stirrer method is considered to be the standard method because it has proven to give the most reliable results in validation studies.

NOTE

Provided equivalence with the method described within this International Standard can be documented, alternative methods can be used for analysis.

PDF Catalog

PDF Pages PDF Title
4 European foreword
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Principle
4.1 General
4.2 Sample size
4.3 Blending/grinding of the muscle sample
4.4 Preparation of the digest fluid
4.5 Digestion of the chopped meat
11 4.6 Filtration of the digest fluid
4.7 Sedimentation of the digest fluid
4.8 Microscopic examination
4.9 Verification of findings
5 Reagents
12 6 Apparatus
13 7 Sampling, labelling, and transport
8 Sample preparation
9 Procedure
9.1 General
9.2 Blending/grinding
14 9.3 Preparation of the digest fluid
9.4 Digestion of the chopped meat in the glass beaker
9.5 Filtration of the digest fluid
15 9.6 Sedimentation of the digest fluid in the separatory funnel
9.7 Collection of the primary and secondary sediment
9.8 Microscopic examination
16 10 Documentation
11 Expression of the results
12 Safety measures
17 Annex A (normative) Sample collection
19 Annex B (normative) Frozen samples
20 Annex C (informative) Artificial digestion magnetic stirrer method
22 Annex D (informative) Example of a laboratory worksheet for recording data when testing pooled samples by digestion assay
23 Bibliography

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BS EN ISO 18743:2015
$142.49