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BS EN ISO 20743:2021

BS EN ISO 20743:2021

$167.15

Textiles. Determination of antibacterial activity of textile products

Published By Publication Date Number of Pages
BSI 2021 42
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This document specifies quantitative test methods to determine the antibacterial activity of all antibacterial textile products including nonwovens.

This document is applicable to all textile products, including cloth, wadding, thread and material for clothing, bedclothes, home furnishings and miscellaneous goods, regardless of the type of antibacterial agent used (organic, inorganic, natural or man-made) or the method of application (built-in, after-treatment or grafting).

This document covers three inoculation methods for the determination of antibacterial activity:

  1. absorption method (an evaluation method in which the test bacterial suspension is inoculated directly onto specimens);

  2. transfer method (an evaluation method in which test bacteria are placed on an agar plate and transferred onto specimens);

  3. printing method (an evaluation method in which test bacteria are placed on a filter and printed onto specimens).

NOTE

Based on the intended application and on the environment in which the textile product is to be used, and also on the surface properties of the textile properties, the user can select the most suitable inoculation method.

This document also specifies the colony plate count method and the adenosine triphosphate (ATP) luminescence method for measuring the enumeration of bacteria.

PDF Catalog

PDF Pages PDF Title
2 National foreword
4 European foreword
7 Foreword
8 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Safety precaution
5 Apparatus
12 5.26 Aluminium foil.
5.27 Reciprocal incubation shaker.
6 Reagents and culture media
6.1 Water
6.2 Tryptone soya broth (TSB)
6.3 Tryptone soya agar (TSA)
13 6.4 Agar for transfer
6.5 Nutrient broth (NB)
6.6 Peptone salt solution
6.7 Physiological saline
6.8 Soybean-Casein Digest Broth with Lecithin & Polysorbate 80 (SCDLP) medium
14 6.9 Dilution buffer for shake-out bacterial suspension
6.10 Neutralizing solution
6.11 Enumeration agar (EA)
15 6.12 Agar for printing
6.13 Cryoprotective solution for bacterial species
6.14 Stock solution of ATP standard reagent
6.15 Buffer solution for ATP luminescent reagent
16 6.16 ATP luminescent reagent
6.17 ATP extracting reagent
6.18 ATP eliminating reagent
17 6.19 SCDLP or other medium for preparing ATP reference solution
6.20 Shake-out physiological saline
7 Reference strains
7.1 Strains
7.2 Restoration and storage of strains
7.2.1 General
7.2.2 Ceramic bead method
18 7.2.3 Glycerol suspension method
8 Test procedures
8.1 Absorption method (see Annex E)
8.1.1 Incubation of test strain
19 8.1.2 Preparation of test inoculum
8.1.3 Preparation of test specimens
20 8.1.4 Test operation
21 8.1.5 Test results
22 8.2 Transfer method (see Annex E)
8.2.1 Preparation of test inoculum
23 8.2.2 Preparation of specimens
8.2.3 Test operation
24 8.2.4 Test results
25 8.3 Printing method (see Annex E)
8.3.1 Incubation of test strain
26 8.3.2 Preparation of test inoculum
8.3.3 Pretreatment of specimen
8.3.4 Test operation
29 8.3.5 Test results
9 Judgement of antibacterial efficacy
10 Test report
31 Annex A (normative) Strain numbers
32 Annex B (normative) Shaking method
33 Annex C (normative) Quantitative measurement by plate count method
34 Annex D (normative) Quantitative measurement by luminescence method
36 Annex E (informative) Testing examples
39 Annex F (informative) Efficacy of antibacterial activity
40 Bibliography

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BS EN ISO 20743:2021
$167.15