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BS EN ISO 20776-1:2020:2024 Edition

BS EN ISO 20776-1:2020:2024 Edition

$142.49

Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices – Broth micro-dilution reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases

Published By Publication Date Number of Pages
BSI 2024 30
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This document describes one reference method, broth micro-dilution, for determination of MICs. The MIC can be a guide for the clinician, and reflects the activity of the drug under the described test conditions, by taking into account other factors, such as drug pharmacology, pharmacokinetics, or bacterial resistance mechanisms. This allows categorisation of bacteria as “susceptible” (S), “intermediate” (I), or “resistant” (R). In addition, MIC distributions can be used to define wild type or non-wild type bacterial populations. Although clinical interpretation of the MIC value is beyond the scope of this document, modifications of the basic method are required for certain antimicrobial agent – bacteria combinations to facilitate clinical interpretation. These modifications are included in a separate annex of this document. It is necessary to compare other susceptibility testing methods (e.g. disc diffusion or diagnostic test devices) with this reference method for validation, in order to ensure comparable and reliable results.

PDF Catalog

PDF Pages PDF Title
2 undefined
5 European foreword
Endorsement notice
8 Foreword
9 Introduction
11 1 Scope
2 Normative references
3 Terms and definitions
13 4 Test procedures
4.1 General
4.2 Medium
4.3 Antimicrobial agents
4.3.1 General
4.3.2 Preparation of stock solutions
14 4.3.3 Preparation of working solutions
4.3.4 Preparation of micro-dilution trays
4.3.5 Storage of micro-dilution trays
15 4.4 Preparation of inoculum
4.4.1 General
4.4.2 Broth culture method
4.4.3 Direct colony suspension method
4.5 Inoculation of micro-dilution trays
16 4.6 Incubation of micro-dilution trays
4.7 Reading results
4.8 Special test situations where the MIC result might give unreliable results
5 Quality control
18 Annex A (informative) Requirements for Mueller-Hinton broth
21 Annex B (informative) Solvents and diluents for making stock solutions of selected antimicrobial agents
26 Annex C (informative) Preparation of working dilutions of antimicrobial agents for use in broth dilution susceptibility tests
27 Annex D (informative) Special test situations
28 Bibliography

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BS EN ISO 20776-1:2020
$142.49