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BS EN ISO 21148:2017

BS EN ISO 21148:2017

$142.49

Cosmetics. Microbiology. General instructions for microbiological examination

Published By Publication Date Number of Pages
BSI 2017 32
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This document gives general instructions for carrying out microbiological examinations of cosmetic products, in order to ensure their quality and safety, in accordance with an appropriate risk analysis (e.g. low water activity, hydro-alcoholic, extreme pH values).

Because of the large variety of products and potential uses within this field of application, these instructions might not be appropriate for some products in every detail (e.g. certain water-immiscible products).

PDF Catalog

PDF Pages PDF Title
2 National foreword
4 European foreword
Endorsement notice
7 Foreword
8 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
4 Premises
4.1 Test areas
10 4.2 Additional areas
4.3 Location of the premises
4.4 Equipping the premises
11 4.5 Maintenance
5 Equipment
5.1 General
5.2 Microbiological cabinets
5.3 Balances
12 5.4 Homogenizer
5.5 pHā€“meter
5.6 Autoclave
5.7 Incubator
5.8 Water baths
5.9 Refrigerator or cold-storage room
5.10 Freezer
13 5.11 Sterilizing oven
5.12 Colony-counting device
5.13 Other equipment
6 Strains of microorganisms
14 7 Personnel
7.1 Competence
7.2 Hygiene
8 Preparation of the apparatus and glassware
8.1 Preparation
8.2 Sterilization
8.2.1 Sterilization by dry heat
15 8.2.2 Sterilization by moist heat
8.3 Disposable apparatus
8.4 Management of clean apparatus and glassware
8.5 Management of sterile apparatus and glassware
8.6 Treatment of contaminated material
8.7 Washing
16 9 Preparation and sterilization of culture media and reagents
9.1 General
9.2 Water
9.3 Preparation of culture media
9.3.1 General
9.3.2 Rehydration
9.3.3 Measurement of pH
9.3.4 Dispensing
17 9.4 Sterilization
9.4.1 General
9.4.2 Sterilization by moist heat
9.4.3 Sterilization by filtration
9.5 Storage
9.5.1 General
9.5.2 Laboratory-prepared culture media and reagents
18 9.5.3 Ready-to-use culture media and reagents
9.6 Melting of agar culture media
9.7 Preparation of Petri dishes
10 Laboratory samples
10.1 General
10.2 Sampling the cosmetic product
10.3 Transport
19 10.4 Receipt and storage
10.5 Handling products and samples
10.6 Conservation and destruction of products
11 Operating practices
11.1 Hygienic precautions during the testing
20 11.2 Preparation of the initial suspension and of sample dilutions
11.2.1 General
11.2.2 Water-miscible product
21 11.2.3 Water-immiscible products
11.3 Counting methods
11.4 Detection methods
12 Expression of results
13 Neutralization of the antimicrobial properties of the product
22 AnnexĀ A (informative) Basic identification techniques
27 AnnexĀ B (informative) Basic techniques for counting and plating
28 AnnexĀ C (informative) Preparation and calibration of inoculums
29 Bibliography

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BS EN ISO 21148:2017
$142.49