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BS EN ISO 22184:2021

BS EN ISO 22184:2021

$167.15

Milk and milk products. Determination of the sugar contents. High performance anion exchange chromatography with pulsed amperometric detection method (HPAEC-PAD)

Published By Publication Date Number of Pages
BSI 2021 34
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This document specifies the quantitative liquid chromatographic determination of specific sugars (galactose, glucose, fructose, sucrose, lactose and maltose) in various milk and milk products, applying arabinose as an internal standard.

The method is applicable to the following dairy matrices: milk, sweetened condensed milk, milk powder, cheese, whey powder, infant formula, milk dessert and yoghurt.

The method does not apply to dairy products containing soy or to the determination of the lactose content in low-lactose milk products at levels below 1 mg/g.

A high performance anion exchange chromatography method in combination with pulsed amperometric detection (HPAEC-PAD) method is applied[ 5][ 3][ 4]. With this method, thirteen different monosaccharides, disaccharides and trisaccharides can be separated: fucose, arabinose, galactose, glucose, fructose, sucrose, lactose, lactulose, maltose, melibiose, trehalose, isomaltulose and maltotriose.

The method is applicable to labelling for the six most important sugars that can be present by nature or by addition in milk and milk products. The method does not apply to sugar contents less than 0,1 %.

PDF Catalog

PDF Pages PDF Title
2 undefined
6 Foreword
9 1 Scope
2 Normative references
3 Terms and definitions
4 Principle
10 5 Reagents
12 6 Apparatus
14 7 Sampling
8 Preparation of the test sample
8.1 General
8.2 Sample preparation of sweetened condensed milk
8.2.1 Samples of recently manufactured products in which no appreciable separation of components can be expected
8.2.2 Samples of older products and samples in which separation of components can be expected
15 9 Procedure
9.1 Sample extraction and clean up
9.1.1 General
9.1.2 Sample extraction and clean-up
17 9.2 Chromatographic analysis
18 10 Calculation and expression of the results
19 11 Precision
11.1 General
11.2 Repeatability
21 11.3 Reproducibility
24 12 Test report
25 Annex A (informative) Precision data
31 Annex B (informative) Accuracy data
33 Bibliography

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BS EN ISO 22184:2021
$167.15