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BS EN ISO 22964:2017

$142.49

Microbiology of the food chain. Horizontal method for the detection of Cronobacter spp.

Published By Publication Date Number of Pages
BSI 2017 32
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This document specifies a horizontal method for the detection of Cronobacter spp.

Subject to the limitations discussed in the introduction, this document is applicable to

  • food products and ingredients intended for human consumption and the feeding of animals, and

  • environmental samples in the area of food production and food handling.

PDF Catalog

PDF Pages PDF Title
2 National foreword
4 European foreword
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Abbreviated terms
5 Principle
5.1 Non-selective pre-enrichment in BPW
5.2 Enrichment in a selective medium (CSB)
5.3 Plating out and identification on chromogenic agar (CCI agar)
5.4 Confirmation
6 Culture media and reagents
7 Equipment and consumables
11 7.10 Pestle and mortar.
8 Sampling
9 Preparation of test sample
10 Procedure (as shown in Annex A)
10.1 Test portion
12 10.2 Pre-enrichment
10.3 Enrichment
10.4 Isolation of presumptive Cronobacter spp.
10.5 Confirmation
10.5.1 General
10.5.2 Purification of colonies
13 10.5.3 Biochemical confirmation
14 10.6 Interpretation of biochemical results
11 Expression of results
12 Performance characteristics of the method
12.1 Interlaboratory study
15 12.2 Sensitivity
12.3 Specificity
12.4 LOD50
13 Test report
16 Annex A (normative) Diagram of the test procedure
17 Annex B (normative) Composition, preparation and performance testing of culture media and reagents
25 Annex C (informative) Distinction of Cronobacter spp. from other genera
27 Annex D (informative) Method validation studies and performance characteristics
30 Bibliography
BS EN ISO 22964:2017
$142.49