BS EN ISO 22964:2017
$142.49
Microbiology of the food chain. Horizontal method for the detection of Cronobacter spp.
Published By | Publication Date | Number of Pages |
BSI | 2017 | 32 |
This document specifies a horizontal method for the detection of Cronobacter spp.
Subject to the limitations discussed in the introduction, this document is applicable to
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food products and ingredients intended for human consumption and the feeding of animals, and
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environmental samples in the area of food production and food handling.
PDF Catalog
PDF Pages | PDF Title |
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2 | National foreword |
4 | European foreword |
6 | Foreword |
7 | Introduction |
9 | 1 Scope 2 Normative references 3 Terms and definitions |
10 | 4 Abbreviated terms 5 Principle 5.1 Non-selective pre-enrichment in BPW 5.2 Enrichment in a selective medium (CSB) 5.3 Plating out and identification on chromogenic agar (CCI agar) 5.4 Confirmation 6 Culture media and reagents 7 Equipment and consumables |
11 | 7.10 Pestle and mortar. 8 Sampling 9 Preparation of test sample 10 Procedure (as shown in Annex A) 10.1 Test portion |
12 | 10.2 Pre-enrichment 10.3 Enrichment 10.4 Isolation of presumptive Cronobacter spp. 10.5 Confirmation 10.5.1 General 10.5.2 Purification of colonies |
13 | 10.5.3 Biochemical confirmation |
14 | 10.6 Interpretation of biochemical results 11 Expression of results 12 Performance characteristics of the method 12.1 Interlaboratory study |
15 | 12.2 Sensitivity 12.3 Specificity 12.4 LOD50 13 Test report |
16 | Annex A (normative) Diagram of the test procedure |
17 | Annex B (normative) Composition, preparation and performance testing of culture media and reagents |
25 | Annex C (informative) Distinction of Cronobacter spp. from other genera |
27 | Annex D (informative) Method validation studies and performance characteristics |
30 | Bibliography |