BS EN ISO 7704:2023

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Water quality. Requirements for the performance testing of membrane filters used for direct enumeration of microorganisms by culture methods

Published By	Publication Date	Number of Pages
BSI	2023	50

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Categories: 07.100.20 - Microbiology of water, BSI

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Description

This document specifies the requirements for the performance testing of membrane filters used for the retention followed by direct enumeration of microorganisms by culture methods. This document is applicable to membrane filters which are used for retention followed by direct enumeration of specific microorganisms on solid media or on other devices containing media, like absorbent pads[19]. This document is not applicable for membrane filters used for concentration and elution or for qualitative methods. These tests are applicable to the membrane filters intended for the microbiological analysis of different types of water, such as: — drinking water, bottled water and other types of water with expected low numbers of microorganisms; — water with expected higher numbers of microorganisms, for example, surface water and process water. These tests are intended to demonstrate the suitability of the whole system (membrane filter together with the culture medium including the filtration step) required for the specific tests described in References [3], [6], [8], [10], [12] and [13]. This document applies to: — manufacturers producing membrane filters; — microbiological laboratories using membrane filters for their own testing or providing these to other end users.

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PDF Pages	PDF Title
2	undefined
4	European foreword Endorsement notice
7	Foreword
9	Introduction
11	1 Scope 2 Normative references 3 Terms and definitions
12	3.1 General terminology
13	3.2 Terminology of performance testing
14	3.3 Terminology for test microorganisms
15	4 Principle 4.1 General 4.1.1 Introduction
16	4.1.2 Batch testing 4.1.3 Supplementary testing
17	4.2 Performance testing 4.2.1 Modules for batch and supplementary testing 4.2.2 Absence of microbial contamination
18	5 Apparatus and glassware 6 Culture media and diluents 7 Preparation of microorganisms for performance testing 7.1 General 7.2 Reference count 7.2.1 Quantitative productivity testing
19	7.2.2 Qualitative selectivity testing 7.2.3 Qualitative specificity testing 7.3 Preparation of a standardized test suspension using a working culture 7.3.1 General 7.3.2 Preparation of the working culture
20	7.3.3 Preparation of a standardized test suspension (inoculum) for the test 7.4 Preparation of a test suspension using reference material 8 Sampling of membrane filters for testing
21	9 Procedure 9.1 General 9.2 Inoculation by spread plate technique 9.2.1 General
22	9.2.2 Inoculation 9.3 Inoculation by membrane filtration technique 9.3.1 General 9.3.2 Inoculation
23	9.4 Incubation and counting 9.5 Test for absence of microbial contamination 10 Calculation, expression and interpretation of results 10.1 General 10.2 Productivity testing
24	10.3 Selectivity testing 10.4 Specificity testing 11 Documentation of test results 11.1 Test report
25	11.2 Information provided by the manufacturer 11.3 Traceability
26	Annex A (normative) Diagram of the procedure for batch testing
27	Annex B (informative) Example of a card for recording test results from batch testing
28	Annex C (informative) Quantitative supplementary testing of membrane filters
31	Annex D (informative) Qualitative supplementary testing of membrane filters
35	Annex E (informative) Practical example of quantitative batch testing and quantitative supplementary testing by the end user
45	Bibliography

Additional information

Status	Definitive
Pages	50
Publication Date	2023-01-27
ISBN	978 0 539 12302 9
Standard Number	BS EN ISO 7704:2023
Title	Water quality. Requirements for the performance testing of membrane filters used for direct enumeration of microorganisms by culture methods
Identical National Standard Of	EN ISO 7704, ISO 7704
Descriptors	Plumbing (water services), Water softening, Water purification, Treatment, Purification (water), Water treatment, Agricultural equipment
Publisher	BSI
Committee	EH/3/4
ICS Codes	07.100.20 - Microbiology of water

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BS EN ISO 7704:2023

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