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BS ISO 16649-1:2018

BS ISO 16649-1:2018

$102.76

Microbiology of the food chain. Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli – Colony-count technique at 44 ° C using membranes and 5-bromo-4-chloro-3-indolyl beta-D-glucuronide

Published By Publication Date Number of Pages
BSI 2018 20
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This document specifies a horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli by colony-count technique after resuscitation using membranes and incubation at 44 °C on a solid medium containing a chromogenic ingredient for detection of the enzyme β-glucuronidase[9][10][13][14][17][18][19][20]. It is applicable to

  • products intended for human consumption,

  • products intended for feeding animals,

  • environmental samples in the area of food production and food handling, and

  • samples from the primary production stage such as animal faeces, dust, and swabs.

WARNING Some strains of Escherichia coli may grow poorly or not at all in media incubated at 44 °C. This includes strains of E. coli O157:H7 and O157:H–. Additionally, some strains of Escherichia coli, notably those belonging to serotype O157:H7, are mostly β-glucuronidase negative[11]. Consequently, some strains of E. coli, including pathogenic ones, will not be detected by this method. β-glucuronidase activity may also be exhibited at 44 °C by certain other members of the Enterobacteriaceae, notably Shigella[15] and Salmonella[16].

PDF Catalog

PDF Pages PDF Title
2 National foreword
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Principle
4.1 Test portion, initial suspension, dilutions and resuscitation step
4.2 Culture on selective medium
4.3 Calculation
5 Culture media and reagents
6 Equipment and consumables
11 7 Sampling
8 Preparation of test sample
9 Procedure
9.1 General
9.2 Test portion, initial suspension and dilutions
12 9.3 Resuscitation
9.4 Transfer to selective medium and incubation
9.5 Counting the colony-forming units (cfu)
9.6 Calculation
10 Expression of results
13 11 Performance characteristics of the method
12 Test report
13 Quality assurance
14 Annex A (normative) Composition and preparation of culture media and reagents
18 Bibliography

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BS ISO 16649-1:2018
$102.76