BS ISO 19007:2018
$167.15
Nanotechnologies. In vitro MTS assay for measuring the cytotoxic effect of nanoparticles
| Published By | Publication Date | Number of Pages |
| BSI | 2018 | 42 |
This document specifies a method for evaluating the effects of nano-objects and their aggregates and agglomerates (NOAA) on cellular viability using the MTS assay. The assay design includes performance requirements and control experiments to identify and manage variability in the assay results.
This document is applicable to the use of a 96-well plate.
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 2 | National foreword |
| 6 | Foreword |
| 7 | Introduction |
| 9 | 1 Scope 2 Normative references 3 Terms and definitions |
| 10 | 4 Symbols and abbreviated terms |
| 11 | 5 Materials 5.1 Cell line 5.2 Assay 5.3 Controls |
| 12 | 6 Apparatus 6.2 Flat bottom 96 multi-well plates. 6.5 96 well plate photometer microtitre plate reader. 6.10 Inverted phase contrast microscope. 6.11 Stereomicroscope. 6.12 Laboratory balance. 6.13 Electronic cell counter or hemocytometer 6.14 Micropipette. 6.15 Vortex mixer. 7 Nanoparticle test sample preparation |
| 13 | 8 Preparations 8.1 General 8.2 Culture medium 8.3 Preparation of cell stock culture |
| 14 | 8.4 Verify viable cell growth 8.5 Verification of plate reader uniformity 8.6 Control preparation 8.6.1 Control description |
| 15 | 8.6.2 CdSO4 stock solution preparation (10mM) 8.6.3 Nanoparticle control suspension preparation 8.7 Precision pipetting 9 Characterization of nanoparticle impact on cell viability 9.1 General |
| 16 | 9.2 Preparation of the cell plate |
| 17 | 9.3 Prepare the nanoparticle dosing plate |
| 19 | 9.4 Expose cells to nanoparticles in culture medium 9.5 Expose cells to MTS Assay |
| 20 | 9.6 Measurement of formazan absorbance 10 Cell viability analysis 11 Interpertation of Assay Results |
| 21 | Annex A (informative) Potential cell lines and assays |
| 22 | Annex B (informative) Example: the MTS assay using the A549 cell line (EMPA-NIST protocol) |
| 31 | Annex C (informative) Example: MTS assay using the RAW 264.7 cell line (IANH protocol) |
| 39 | Bibliography |
