BS ISO 4134:2021

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Meat and meat products. Determination of L-(+)-glutamic acid content. Reference method

Published By	Publication Date	Number of Pages
BSI	2021	24

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Categories: 67.120.10 - Meat and meat products, BSI

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Description

This document specifies the spectrophotometer method and the light absorption microplate reader method for the determination of the free L-(+)-glutamic acid content of meat and meat products.

This document is applicable to meat and meat products, including livestock and poultry products.

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PDF Pages	PDF Title
2	National foreword
6	Foreword
9	1 Scope 2 Normative references 3 Terms and definitions 4 Principle
10	5 Sampling 6 Preparation of test sample 7 Test method of spectrophotometer 7.1 Reagents
11	7.1.6 Solution R123, the mixed solution of solution R1, solution R2 and solution R3.
12	7.2 Apparatus 7.2.5 Constant temperature drying box. 7.2.6 pH-meter.
13	7.3 Procedure 7.3.1 General 7.3.2 Test portion 7.3.3 Preparation of extract 7.3.4 Determination
14	7.4 Calculation and results 7.4.1 Absorbance difference of L-(+)-glutamic acid standard solution
15	7.4.2 Absorbance difference of L-(+)-glutamic acid test solution 7.4.3 Formula of linear regression of L-(+)-glutamic acid standard curve 7.4.4 L-(+)-glutamic acid concentration of the test solution 7.4.5 L-(+)-glutamic acid content of test sample
16	7.5 Precision 7.5.1 Interlaboratory test 7.5.2 Repeatability 7.5.3 Reproducibility 7.6 Detection limit 8 Test method of light absorption microplate reader 8.1 Reagents 8.2 Apparatus
17	8.3 Procedure 8.3.1 General 8.3.2 Extraction of L-(+)-glutamic acid in the test portion 8.3.3 Determination
18	8.4 Calculation and results 8.4.1 Absorbance difference for L-(+)-glutamic acid standard solution 8.4.2 Absorbance difference for L-(+)-glutamic acid test solution
19	8.4.3 Formula of linear regression for L-(+)-glutamic acid standard curve 8.4.4 L-(+)-glutamic acid concentration of the test solution 8.4.5 L-(+)-glutamic acid content of test sample
20	8.5 Precision 8.5.1 Interlaboratory test 8.5.2 Repeatability 8.5.3 Reproducibility 8.6 Detection limit 9 Test report
21	Annex A (informative) Safety practices

Additional information

Status	Definitive
Pages	24
Publication Date	2021-07-30
ISBN	978 0 539 06212 0
Standard Number	BS ISO 4134:2021
Title	Meat and meat products. Determination of L-(+)-glutamic acid content. Reference method
Identical National Standard Of	ISO 4134
Descriptors	Meat products, Animal products, Meat, Amino acids, Glutamic acid
Publisher	BSI
Committee	W/-
ICS Codes	67.120.10 - Meat and meat products

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BS ISO 4134:2021

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