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BS ISO 4214:2022

BS ISO 4214:2022

$189.07

Milk and milk products. Determination of amino acids in infant and adult/paediatric nutritional formulas and dairy products

Published By Publication Date Number of Pages
BSI 2022 46
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PDF Catalog

PDF Pages PDF Title
2 National foreword
4 Forewords
7 1 Scope
2 Normative references
3 Terms and definitions
4 Principle
8 5 Reagents
9 6 Reagents and standard preparation
6.1 General
10 6.2 Norvaline (Nva) internal standards
6.3 Cystine calibration standards
11 6.4 Amino acid (AA) calibration standards (with exception of cystine)
6.5 Chromatography solvents (mobile phases)
6.5.1 Eluent A (Solvent A).
6.5.2 Eluent B (Solvent B).
6.5.3 Wash solvents.
12 7 Apparatus
7.4 Vortex mixer.
8 Sample analysis
8.1 Sample preparation
13 8.2 Cystine calibration standards preparation
8.3 Hydrolysis (of samples and cystine standards)
8.4 Neutralization and dilution (of samples and cystine standards)
14 8.5 Amino acids calibration standards preparation (not needed to be acid hydrolysed)
8.6 Derivatization (of samples, cystine standards and amino acids standards)
8.7 UHPLC separation
15 8.8 Peak identification and integration
16 9 Calculation and expression of results
9.1 Calibration curve
9.2 Amino acid calculation
17 10 Precision
10.1 General
10.2 Repeatability
10.3 Reproducibility
24 11 Test report
25 Annex A (informative) Precision data
43 Annex B (informative) Chromatogram examples
44 Bibliography

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BS ISO 4214:2022
$189.07