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BSI 21/30422060 DC:2021 Edition

BSI 21/30422060 DC:2021 Edition

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BS EN 17641. Foodstuffs. Multimethod for the determination of aflatoxins, deoxynivalenol, fumonisins, ochratoxin A, T-2 toxin, HT-2 toxin and zearalenone by LC-MS/MS

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BSI 2021 50
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This document describes an isotope dilution method for the quantitative determination of aflatoxins B1, B2, G1, G2 and M1 (AFB1, AFB2, AFG1, AFG2 and AFM1), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZEN), T-2 and HT-2 toxins (T-2 and HT-2) and fumonisins B1 and B2 (FB1 and FB2) in foods by liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS). A specific immunoaffinity column (IAC) clean-up is needed for aflatoxins (AFs) and OTA in infant foods (e.g. infant cereals, milk-based powders), in spices, in dried fruits and in nuts. The method has been validated through an intercollaborative study on different commodity groups: cereals and cereal-based products including food for infant and young children, nuts, spices, dried fruits and milk powder. The ranges of concentrations of each mycotoxin in these naturally contaminated and/or spiked food samples were: – aflatoxin B1: 0,0857 µg/kg – 11,4 µg/kg; – aflatoxin B2: 0,0792 µg/kg – 12,5 µg/kg; – aflatoxin G1: 0,0628 µg/kg – 20,9 µg/kg; – aflatoxin G2: 0,0520 µg/kg – 15,0 µg/kg; – aflatoxin M1: 0,0342 µg/kg – 0,110 µg/kg; – ochratoxin A: 0,448 µg/kg – 17,2 µg/kg; – deoxynivalenol: 45,2 µg/kg – 743 µg/kg; – zearalenone: 9,57 µg/kg – 131 µg/kg; – T-2 toxin: 10,3 µg/kg – 57,9 µg/kg; – HT-2 toxin: 9,50 µg/kg – 81,8 µg/kg; – fumonisin B1: 31,1 µg/kg – 4 262 µg/kg; – fumonisin B2: 44,2 µg/kg – 1 299 µg/kg. The measuring ranges of the method for each mycotoxin/matrix combination are given in Table 7.

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BSI 21/30422060 DC
$13.70