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BSI 22/30422334 DC 2022

BSI 22/30422334 DC 2022

$13.70

BS ISO 24421. Biotechnology. Minimum requirements for optical signal measurements in photometric methods for biological samples

Published By Publication Date Number of Pages
BSI 2022 43
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PDF Catalog

PDF Pages PDF Title
1 30422334-NC.pdf
3 ISO_DIS 24421 ed.1 – id.78742 Enquiry PDF (en).pdf
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
12 4 Principles
4.1 General
13 4.2 Instruments and measurements
14 4.3 Optical references
5 Minimum requirements to support optical signal measurement
5.1 Elements of photometric methods
15 5.2 ​Verification of optical signal measurement instruments
5.2.1 Optical references
5.2.2 Dynamic range
16 5.2.3 Background signals
5.3 Optical signal measurement of biological samples
5.3.1 Optical signal measurement
5.3.2 Calibration curve
17 5.3.3 Photometric methods
5.3.4 Personnel
5.4 Data analysis and reporting
19 Annex€A (informative) Principles of bioluminescence, chemiluminescence, fluorescence and absorption
22 Annex€B (informative) List of optical references, optical signal measurement instruments, and€representative photometric methods
23 Annex€C (informative) List of relevant standards describing representative methods by€means of€optical signal measurements
24 Annex€D (informative) Example of qualification of luminometer using LED reference light source
26 Annex€E (informative) Example of application of reference light source for comparison measurements of bioluminescent sample using luminometers
28 Annex€F (informative) Example of determination of well-to-well crosstalk in multi-well plates
30 Annex€G (informative) Examples of dynamic range determination of luminometer
33 Annex€H (informative) Example of construction of calibration curve and dynamic range determination of fluorescence plate reader
35 Annex€I (informative) Example of dynamic range determination of a flow cytometer
37 Annex€J (informative) Example of calibration of reference light sources and luminometers
40 Annex€K (informative) Examples of spectral properties of photodetectors
42 Bibliography

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BSI 22/30422334 DC 2022
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