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BSI 23/30466937 DC 2023

BSI 23/30466937 DC 2023

$13.70

BS ISO 24479 Biotechnology — Cellular morphological analysis — General requirements and considerations for cell morphometry to quantify cell morphological features

Published By Publication Date Number of Pages
BSI 2023 51
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PDF Catalog

PDF Pages PDF Title
7 Foreword
8 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
13 4 Abbreviations
5 General Concept
5.1 Cell morphology
5.2 Steps for cell morphometry
6 Target of interest (TOI)
14 7 Image capture
7.1 Microscopic observation method
7.1.1 General
7.1.2 Cell properties to be observed
15 7.1.3 Sample preparation
17 7.2 Microscope system and its settings
7.2.1 General
19 7.2.2 Light source
7.2.3 Objective lens and condenser lens
7.2.4 Components in optical path
20 7.2.5 Image capture device
7.2.6 Image data
7.2.7 Environmental conditions
7.2.8 Observing position
7.2.9 Image capturing conditions
21 7.3 Execution of image capture
7.3.1 Single image capture
7.3.2 Multiple image capture
8 Segmentation of the TOI
22 9 Quantification
9.1 General
9.2 Procedure to quantify morphological features of segmented object
10 Qualification of Measurement
11 Reporting
11.1 General
23 11.2 Reporting of sample properties and sample preparation
11.3 Reporting of the microscopic observation method
24 11.4 Reporting of the image data, image pre-processing, and image analysis for morphometric analysis
11.5 Reporting of the morphological features for morphometric analysis
25 Annex€A (informative) Check sheet regarding selection of contrast-enhancing techniques for optical microscopy
27 Annex€B (informative) Check sheet regarding selection of microscope system
28 Annex€C (informative) List of morphological feature parameters, description and formula – Cell shape, size –
36 Annex€D (informative) List of morphological feature parameters, description and formula – Cell texture –
41 Annex€E (informative) Points to consider when acquiring phase-contrast images of cells suitable for image analysis
51 Bibliography

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BSI 23/30466937 DC 2023
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