BSI PD CEN/TS 17804:2022

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Organic, organo-mineral and inorganic fertilizers. Detection of Enterocococaceae

Published By	Publication Date	Number of Pages
BSI	2022	20

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This document specifies a method for the detection and enumeration of Enterococcaceae in fertilizers of the following Product Function Categories (PFCs) of EU fertilizing products, as described in Regulation (EU) 2019/1009 [1]: • PFC 1(A): Organic fertilizer; • PFC 1(B): Organo-mineral fertilizer; • PFC 1(C): Inorganic fertilizer, which contains more than 1 % by mass of organic carbon, other than organic carbon from chelating or complexing agents, nitrification inhibitors, denitrification inhibitors or urease inhibitors, coating agents, urea or calcium cyanamide. The present method was validated on products known as present on the market in April 2021 and conform to Regulation (EU) 2019/1009 [1] that are inorganic fertilizers with more than 1 % of organic carbon such as poultry manure and struvite with low level of organic matter. In case that other products would be developed having other physical and chemical characteristics, it might become necessary to develop different methods to correctly account for pathogens they might contain. This document specifies a colony-count technique on selective media, Slanetz Bartley agar or Bile Esculin Azide agar, respectively. The method is based on EN ISO 7899 2:2000.

PDF Catalog

PDF Pages	PDF Title
2	undefined
7	1 Scope 2 Normative references 3 Terms and definitions
8	4 Principle
9	5 Reagents 5.1 General 5.2 Diluents 5.2.1 General 5.2.2 Basic phosphate buffer 5.2.3 Double-buffered phosphate buffer 5.3 Selective media 5.3.1 Slanetz Bartley agar
10	5.3.2 Bile Esculin Azide agar 6 Equipment and consumables
11	7 Sampling 8 Preparation of test sample 9 Procedure (see Figure A.1 in Annex A) 9.1 Preparation of the initial suspension and decimal dilutions 9.2 Inoculation and incubation 9.2.1 General
12	9.2.2 Procedure 9.3 Enumeration of colonies 9.4 Confirmation (optional) 9.4.1 General 9.4.2 Confirmation on the second selective medium
13	9.4.3 Catalase Test 10 Expression of results
14	11 Test report
15	Annex A (normative)Diagram of the procedure
16	Annex B (normative)Composition and preparation of culture media and reagents B.1 General B.2 Basic phosphate buffer B.2.1 Composition B.2.2 Preparation
17	B.3 Double-buffered phosphate buffer B.3.1 Composition B.3.2 Preparation B.4 Slanetz Bartley agar B.4.1 Composition B.4.2 Preparation
18	B.5 Bile Esculin Azide agar B.5.1 Composition B.5.2 Preparation