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BSI PD ISO/TS 12788:2022

BSI PD ISO/TS 12788:2022

$142.49

Rapeseed. Determination of glucosinolate content. Spectrometric method for total glucosinolates by glucose release

Published By Publication Date Number of Pages
BSI 2022 30
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PDF Catalog

PDF Pages PDF Title
2 National foreword
7 Foreword
8 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
10 5.1 Hydrochloric acid (2 M).
5.2 Phenol tungstate reagent.
5.3 Glucose determination reagents.
5.4 Glucose oxidase/peroxidase assay.
5.5 Sodium phosphate buffer.
5.6 Glucose oxidase reagent.
5.7 Peroxidase colour reagent.
5.8 Glucose hexokinase/Glucose-6-phosphate dehydrogenase glucose assay.
11 5.9 Myrosinase solution.
5.10 Sodium hydroxide (0,5 M).
5.11 Sodium acetate buffer (0,2 M).
5.12 Tris-HCl buffer.
5.13.1 Stock solution.
5.13.2 Calibration samples.
6 Apparatus
12 7 Sampling
13 8 Preparation of the test sample
8.1 Reduction of test sample to laboratory sample
8.2 Moisture and volatile matter content
8.3 Grinding
9 Procedure
9.1 Test portion
9.2 Extraction of glucosinolates
9.2.1 General
9.2.2 Principle
9.2.3 Operating mode
14 9.3 Hydrolysis and collection of glucose from glucosinolates by myrosinase and column chromatography
9.4 Glucose determination
9.4.1 General
9.4.2 Glucose oxidase/peroxidase glucose assay (5.4)
15 9.4.3 Glucose hexokinase/glucose-6-phosphate dehydrogenase assay (5.8)
16 10 Calculation of glucosinolate content
17 11 Expression of results
12 Precision
12.1 Interlaboratory test
12.2 Repeatability
12.3 Reproducibility
12.4 Critical difference
12.4.1 General
12.4.2 Comparison of two groups of measurements in one laboratory
12.4.3 Comparison of two groups of measurements in two laboratories
18 13 Test report
19 Annex A (informative) Extraction and purification of myrosinase from Sinapis alba L., also called white or yellow mustard
23 Annex B (informative) Determination of recovery of glucosinolates
26 Annex C (informative) Results of the interlaboratory test
28 Bibliography

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BSI PD ISO/TS 12788:2022
$142.49