🔥🔥 Don’t Miss Out on Our End of Autumn Sale. If you have any questions, feel free to click the bottom right corner to contact our customer service..

Concat us[email protected]
Shopping Cart

No products in the cart.

🔍
BSI PD ISO/TS 20224-1:2020

BSI PD ISO/TS 20224-1:2020

$142.49

Molecular biomarker analysis. Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR – Bovine DNA detection method

Published By Publication Date Number of Pages
BSI 2020 26
PDF Format Searchable Printable Preview Now
Guaranteed Safe Checkout
Category:

If you have any questions, feel free to reach out to our online customer service team by clicking on the bottom right corner. We’re here to assist you 24/7.
Email:[email protected]

This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of bovine-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of bovine material derived from cattle including taurine (Bos taurus) and zebu (Bos indicus). The assay also detects the species bison (Bison bison) and yak (Bos mutus).

The target sequence is a partial fragment of the bovine nuclear beta actin gene in chromosome 25 (i.e. GenBank accession number NC_037352.1)[ 1][ 2][ 3], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ? 95 % replicability at this concentration (LOD95 %).

PDF Catalog

PDF Pages PDF Title
2 undefined
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Scientific basis
5 Reagents and materials
5.1 General
5.2 PCR reagents
11 6 Apparatus
7 Procedure
7.1 Preparation of the test portion/sample
7.2 Preparation of DNA extracts
7.3 PCR setup
7.3.1 Reaction mixes
12 7.3.2 PCR controls
7.3.3 Real-time PCR thermocycler plate set-up
7.4 Temperature-time programme
8 Accept/reject criteria
8.1 General
13 8.2 Identification
9 Validation status and performance criteria
9.1 General
9.2 Robustness
14 9.3 Reproducibility
9.4 Sensitivity
17 9.5 Specificity
19 10 Test report
20 Annex A (informative) BlastN 2.9.0 results for query of GenBank refseq_genomes (6 databases)
24 Bibliography

Related products

BSI PD ISO/TS 20224-1:2020
$142.49