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BSI PD ISO/TS 21569-2:2021

BSI PD ISO/TS 21569-2:2021

$102.76

Molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products – Construct-specific real-time PCR method for detection of event FP967 in linseed and linseed products

Published By Publication Date Number of Pages
BSI 2021 18
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This document specifies a procedure for the detection of a DNA sequence present in a genetically modified linseed (Linum usitatissimum) line (event FP967, also named as “CDC Triffid”). For this purpose, extracted DNA is used in a real-time PCR and the genetic modification (GM) is specifically detected by amplification of a 105 bp DNA sequence representing the transition between the nopaline synthase gene terminator (Tnos) from Agrobacterium tumefaciens and the dihydrofolate reductase gene (dfrA1) from a Class 1 integron of Escherichia coli.

The method described is applicable for the analysis of DNA extracted from foodstuffs. It can also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix for the purpose of analysis.

PDF Catalog

PDF Pages PDF Title
2 National foreword
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Principle
5 Reagents and materials
5.1 PCR reagents
5.1.4 Standard DNA for calibration
11 6 Apparatus
6.1 General
6.2 PCR device
7 Sampling
8 Procedure
8.1 Test sample preparation
8.2 Preparation of the DNA extracts
8.3 DNA extraction
8.4 PCR setup
12 8.5 Temperature–time programme
9 Accept/reject criteria
9.1 General
13 9.2 Identification
10 Validation status and performance criteria
10.1 Robustness of the method
10.2 Intra-laboratory trial
10.3 Collaborative trial
15 10.4 Sensitivity
10.5 Specificity
16 11 Test report
17 Bibliography

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BSI PD ISO/TS 21569-2:2021
$102.76