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BSI PD ISO/TS 21569-3:2020

BSI PD ISO/TS 21569-3:2020

$102.76

Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products – Construct-specific real-time PCR method for detection of P35S-pat-sequence for screening for genetically modified organisms

Published By Publication Date Number of Pages
BSI 2020 22
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This document describes a procedure for the detection of the DNA transition sequence between the 35S promotor (P35S) from Cauliflower mosaic virus and a modified phoshinothricin-acetyltransferase gene (pat) from Streptomyces viridochromogenes. The P35S-pat construct is frequently found in genetically modified plants with tolerance for phosphinothricin-containing herbicides. The P35S-pat construct specific method is based on a real-time PCR and can be used for qualitative and quantitative screening purposes. For identification and quantification of a specific event, a follow-up analysis can be carried out.

This document is applicable to the analysis of DNA extracted from foodstuffs. It can also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate quantity and quality of amplifiable DNA from the relevant matrix.

PDF Catalog

PDF Pages PDF Title
2 undefined
6 Foreword
7 1 Scope
2 Normative references
3 Terms and definitions
8 4 Principle
5 Reagents and materials
5.1 General
5.2 PCR reagents
9 6 Apparatus
7 Procedure
7.1 Test sample procedure
7.2 Preparation of the DNA extracts
7.3 PCR setup
10 7.4 Temperature-time programme
8 Accept/reject criteria
8.1 General
8.2 Identification
11 8.3 Calculation of P35S-pat copy numbers
9 Validation status and performance criteria
9.1 Robustness of the method
9.2 Collaborative trial for determination of LOD
12 9.3 Collaborative trial for quantification of the P35S-pat construct in rapeseed
13 9.4 Sensitivity
9.5 Specificity
14 10 Test report
15 Annex A (informative) GenBank BLASTN Search
17 Annex B (informative) Joint Research Commission GMO-Matrix
20 Bibliography

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BSI PD ISO/TS 21569-3:2020
$102.76