BSI PD ISO/TS 21872-2:2020
$167.15
Microbiology of the food chain. Horizontal method for the determination of Vibrio spp. – Enumeration of total and potentially enteropathogenic Vibrio parahaemolyticus in seafood using nucleic acid hybridization
| Published By | Publication Date | Number of Pages |
| BSI | 2020 | 36 |
This document specifies a method for the direct enumeration of potentially enteropathogenic V. parahaemolyticus (tdh and/or trh positive) and/or the enumeration of total V. parahaemolyticus in seafood.
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 2 | undefined |
| 6 | Foreword |
| 7 | Introduction |
| 9 | 1 Scope 2 Normative references 3 Terms and definitions |
| 10 | 4 Principle 4.1 General 4.2 Plating 4.3 Preparation of membranes 4.4 Hybridization 4.5 Detection |
| 11 | 5 Culture media and reagents 5.1 General 5.2 Hybridization reagents 5.2.1 Hybridization buffer 5.2.2 Digoxigenin-labelled oligoprobes 5.2.3 Control materials 6 Equipment and consumables |
| 12 | 7 Sampling 8 Procedure 8.1 General 8.2 Preparation of the sample 8.3 Plating 8.4 Preparation of process control 8.5 Preparation of membranes 8.5.1 Colony lift |
| 13 | 8.5.2 Cross-linking of DNA to the membrane 8.5.3 Proteinase K treatment 8.6 Hybridization 8.6.1 General |
| 14 | 8.6.2 Hybridization of tdh and trh probes 8.6.3 Colorimetric detection |
| 15 | 8.6.4 Enumeration of tdh and/or trh positive colonies 8.6.5 Isolation of colonies (optional) 8.6.6 Hybridization of toxR probe |
| 16 | 8.6.7 Colorimetric detection 8.6.8 Enumeration of toxR positive colonies 9 Interpretation of the results |
| 17 | 10 Expression of results 11 Test report |
| 18 | Annex A (normative) Flow diagram for the enumeration of total and potentially enteropathogenic Vibrio parahaemolyticus in seafood using nucleic acid hybridization |
| 21 | Annex B (normative) Culture media and reagents |
| 27 | Annex C (informative) Hybridization of tdh, trh and toxR genes of V. parahaemolyticus |
| 35 | Bibliography |
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