BSI PD ISO/TS 22082:2020
$102.76
Nanotechnologies. Assessment of nanomaterial toxicity using dechorionated zebrafish embryo
| Published By | Publication Date | Number of Pages |
| BSI | 2020 | 22 |
This document specifies a method for rapidly assessing nanomaterial toxicity (fish early life stage, 0 HPF to 120 HPF). It includes information on the importance of acellular chorion removal, detailed chorion removal procedures, and a complete protocol for the toxicity assessment of nanomaterials using dechorionated zebrafish embryos. The focus of this document is on testing nanomaterial toxicity.
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 2 | undefined |
| 6 | Foreword |
| 7 | Introduction |
| 8 | 1 Scope 2 Normative references 3 Terms and definitions |
| 9 | 4 Abbreviated terms 5 Materials |
| 10 | 6 Apparatus 6.1 Technical equipment 6.2 Analytical instruments |
| 11 | 7 Procedures 7.1 Culture 7.1.1 Zebrafish strain 7.1.2 Feeding regime 7.1.3 Photoperiod 7.1.4 Temperature and pH 7.1.5 Tank material 7.2 Spawning stimulation |
| 12 | 7.3 Dechorionation of embryos 7.3.1 Methods 7.3.2 Enzymatic dechorionation method |
| 13 | 7.4 Preparation of nanomaterials stock solution 7.5 Testing concentrations 7.6 Dispersion of nanomaterials 7.7 Positive chemical: 3,4-dichloroaniline |
| 14 | 7.8 Test methods 7.8.1 Water quality measurement 7.8.2 Preparation of dechorionated embryos 7.8.3 Exposure 7.8.4 Endpoints observation |
| 15 | 7.9 Data analysis 8 Test report 8.1 Test procedure 8.2 Information to be included in the report 8.2.1 Test nanomaterials 8.2.2 Test species 8.2.3 Materials and apparatus |
| 16 | 8.2.4 Maintenance 8.2.5 Test design 8.2.6 Test conditions 8.2.7 Results |
| 17 | Annex A (informative) Mechanical dechorionation method |
| 18 | Annex B (informative) Zebrafish embryo spawning procedures |
| 19 | Annex C (informative) Results validation |
| 20 | Bibliography |
