This document specifies a test method and the minimum requirements for bactericidal activity of chemical disinfectant and antiseptic products that form a homogeneous, physically stable preparation when diluted with hard water or – in the case of ready-to-use products – with water. Products can only be tested at a concentration of 80\u00c3?\u00c2 % or less, as some dilution is always produced by adding the test organisms and interfering substance.<\/p>\n
The method described is intended to determine the activity of commercial formulations or active substances under the conditions in which they are used. This document applies to products that are used for equipment disinfection by immersion, surface disinfection by wiping, spraying, flooding or other means and teat disinfection in the veterinary area \u00c3\u00a2?? e.g. in the breeding, husbandry, production, veterinary care facilities, transport and disposal of all animals except when in the food chain following death and entry into processing industry. This document also applies to products used for teat disinfection in these veterinary areas.<\/p>\n
This method is not applicable to evaluate the activity of hand hygiene products. For these products reference is made to EN\u00c3?\u00c2 14885<\/span><\/span>, which specifies in detail the relationship of the various tests to one another and to \u00c3\u00a2??use recommendations\u00c3\u00a2??.<\/p>\n This method corresponds to a phase 2 step 1 test.<\/p>\n<\/div>\n Chemical disinfectants and antiseptics. Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in the veterinary area. Test method and requirements (phase 2, step 1)<\/b><\/p>\nPDF Catalog<\/h4>\n
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\n PDF Pages<\/th>\n PDF Title<\/th>\n<\/tr>\n \n 2<\/td>\n National foreword <\/td>\n<\/tr>\n \n 8<\/td>\n 1 Scope
2 Normative references
3 Terms and definitions
4 Requirements <\/td>\n<\/tr>\n\n 10<\/td>\n 5 Test method
5.1 Principle
5.2 Materials and reagents
5.2.1 Test organisms <\/td>\n<\/tr>\n\n 11<\/td>\n 5.2.2 Culture media and reagents
5.2.2.1 General
5.2.2.2 Water
5.2.2.3 Tryptone Soya Agar (TSA) <\/td>\n<\/tr>\n\n 12<\/td>\n 5.2.2.4 Diluent
5.2.2.5 Neutralizer
5.2.2.6 Rinsing liquid (for membrane filtration)
5.2.2.7 Hard water for dilution of products <\/td>\n<\/tr>\n\n 13<\/td>\n 5.2.2.8 Interfering substance
5.3 Apparatus and glassware
5.3.1 General <\/td>\n<\/tr>\n\n 14<\/td>\n 5.3.2 Usual microbiological laboratory equipment and, in particular, the following: <\/td>\n<\/tr>\n \n 15<\/td>\n 5.4 Preparation of test organism suspensions and product test solutions
5.4.1 Test organism suspensions (test and validation suspension)
5.4.1.1 General
5.4.1.2 Preservation and stock cultures of test organisms
5.4.1.3 Working culture of test organisms
5.4.1.4 Test suspension (N) <\/td>\n<\/tr>\n\n 16<\/td>\n 5.4.1.5 Validation suspension (Nv)
5.4.1.6 Incubation and counting of the test and the validation suspensions
5.4.2 Product test solutions <\/td>\n<\/tr>\n\n 17<\/td>\n 5.5 Procedure for assessing the bactericidal activity of the product
5.5.1 General
5.5.1.1 Experimental conditions
5.5.1.2 Choice of test method (dilution-neutralization or membrane filtration)
5.5.1.3 General instructions for validation and control procedures <\/td>\n<\/tr>\n\n 18<\/td>\n 5.5.1.4 Equilibration of temperature
5.5.1.5 Precautions for manipulation of test organisms
5.5.2 Dilution-neutralization method
5.5.2.1 General
5.5.2.2 Test \u201cNa\u201d \u2013 determination of bactericidal concentrations <\/td>\n<\/tr>\n\n 19<\/td>\n 5.5.2.3 Experimental conditions control A \u2013 validation of the selected experimental conditions and\/or verification of the absence of any lethal effect in the test conditions
5.5.2.4 Neutralizer control B \u2013 verification of the absence of toxicity of the neutralize
5.5.2.5 Method validation C \u2013 dilution-neutralization validation <\/td>\n<\/tr>\n\n 20<\/td>\n 5.5.2.6 Incubation and counting of the test mixture and the control and validation mixtures
5.5.3 Membrane filtration method
5.5.3.1 General
5.5.3.2 Test Na \u2013 determination of the bactericidal concentrations <\/td>\n<\/tr>\n\n 21<\/td>\n 5.5.3.3 Experimental conditions control A \u2013 validation of the selected experimental conditions and\/or verification of the absence of any lethal effect in the test conditions
5.5.3.4 Filtration control B \u2013 validation of the filtration procedure
5.5.3.5 Method validation C \u2013 validation of the membrane filtration method or counting of the bacteria on the membranes which have previously been in contact with the mixture of product and interfering substance
5.5.3.6 Incubation and counting of test mixture and the control and the validation mixtures <\/td>\n<\/tr>\n\n 22<\/td>\n 5.6 Experimental data and calculation
5.6.1 Explanation of terms and abbreviations
5.6.1.1 Overview of the different suspensions and test mixtures
5.6.1.2 Vc values
5.6.2 Calculation
5.6.2.1 General <\/td>\n<\/tr>\n\n 23<\/td>\n 5.6.2.2 Determination of Vc values
5.6.2.3 Calculation of N and N0 <\/td>\n<\/tr>\n\n 24<\/td>\n 5.6.2.4 Calculation of Na <\/td>\n<\/tr>\n \n 25<\/td>\n 5.6.2.5 Calculation of Nv and Nv0
5.6.2.6 Calculation of A, B and C
5.7 Verification of methodology
5.7.1 General
5.7.2 Control of weighted mean counts <\/td>\n<\/tr>\n\n 26<\/td>\n 5.7.3 Basic limits
5.8 Expression of results and precision
5.8.1 Reduction
5.8.2 Control of active and non-active product test solution (5.4.2)
5.8.3 Limiting test organism and bactericidal concentration
5.8.4 Precision, replicates <\/td>\n<\/tr>\n\n 27<\/td>\n 5.9 Interpretation of results – conclusion
5.9.1 General
5.9.2 Bactericidal activity for surface disinfection products
5.9.3 Bactericidal activity for teat disinfection products
5.10 Test report <\/td>\n<\/tr>\n\n 29<\/td>\n Annex\u00a0A (informative)Referenced strains in national collections <\/td>\n<\/tr>\n \n 31<\/td>\n Annex\u00a0B (informative)Examples of neutralizers of the residual antimicrobial activity of chemical disinfectants and antiseptics and rinsing liquids <\/td>\n<\/tr>\n \n 33<\/td>\n Annex\u00a0C (informative)Dilution-neutralization method <\/td>\n<\/tr>\n \n 38<\/td>\n Annex\u00a0D (informative)Example of a typical test report <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" \n\n
\n Published By<\/td>\n Publication Date<\/td>\n Number of Pages<\/td>\n<\/tr>\n \n BSI<\/b><\/a><\/td>\n 2019<\/td>\n 44<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":279539,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[1029,2641],"product_tag":[],"class_list":{"0":"post-279534","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-71-100-35","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/279534","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/279539"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=279534"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=279534"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=279534"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}