ASTM-F895 2006

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F895-84(2006) Standard Test Method for Agar Diffusion Cell Culture Screening for Cytotoxicity

Published By	Publication Date	Number of Pages
ASTM	2006	5

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Categories: 07.100.10 - Medical microbiology, ASTM

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Description

1.1 This test method is appropriate for materials in a variety of shapes and for materials which are not necessarily sterile. This test method would be appropriate in situations where the amount of material is limited. For example, small devices or powders could be placed on the agar and the presence of a zone of inhibition of cell growth could be examined.

1.1.1 This test method is not appropriate for leachables which do not diffuse through agar or agarose.

1.1.2 While the agar layer can act as a cushion to protect the cells from the specimen, there may be materials which are sufficiently heavy to compress the agar and prevent diffusion or to cause mechanical damage to the cells. This test method would not be appropriate for these materials.

1.2 The L-929 cell line was chosen because it has a significant history of use in assays of this type. This is not intended to imply that its use is preferred, only that the L-929 is an established cell line, well-characterized and readily available, that has demonstrated reproducible results in several laboratories.

1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

PDF Catalog

PDF Pages	PDF Title
1	Scope Referenced Documents Summary of Test Method Significance and Use
2	Apparatus Reagents Cell Culture Control Materials General Technique Specimen Preparation
3	Cell Culture Maintenance Cell Layer Preparation Test Procedure
4	Evaluation of Results Report Precision and Bias Keywords X1. RATIONALE X1.1 TABLE 1 TABLE 2
5	REFERENCES

Additional information

Standard Title	F895-84(2006) Standard Test Method for Agar Diffusion Cell Culture Screening for Cytotoxicity
Published Code	ASTM
Publication Date	2006
Pages Count	5
ICS Codes	07.100.10 - Medical microbiology

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ASTM-F895 2006

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