BS EN 13368-2:2017 – TC:2020 Edition
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Tracked Changes. Fertilizers. Determination of chelating agents in fertilizers by chromatography – Determination of Fe chelated by [o,o] EDDHA, [o,o] EDDHMA and HBED, or the amount of chelating agents, by ion pair chromatography
| Published By | Publication Date | Number of Pages |
| BSI | 2020 | 65 |
This European Standard specifies a method for the chromatographic determination of the iron chelated by each individual ortho(hydroxy)-ortho(hydroxy) isomer of the chelating agents [o,o] EDDHA, [o,o] EDDHMA and by HBED in fertilizers containing one or more of these substances, except for [o,o] EDDHMA and HBED mixes. The method allows the identification and the determination of the total concentration of water soluble iron chelates of these chelating agents. Also, after derivatization with Fe, the soluble amount of the chelating agents can be determined when other micro-nutrients, beside Fe are present in fertilizers containing [o,o] EDDHA, [o,o] EDDHMA or HBED. This method is applicable to EC fertilizers covered by Regulation (EC) No 2003/2003 [4]. It is applicable to a mass fraction of the metal chelated of at least 0,625 %. NOTE 1 The substances EDDHA (ethylenediamine-N,N’-di[(hydroxyphenyl)acetic acid] and EDDHMA (ethylenediamine-N,N’-di[(hydroxymethylphenyl)acetic acid] exist as several different isomeric forms. Positional isomers for the hydroxyl or methyl groups (in ortho, meta, and para positions) as well as stereo isomers (meso and dl-racemic forms) are known. Both meso and dl-racemic forms of the [ortho,ortho] EDDHA and [ortho,ortho] EDDHMA are positional isomers for the hydroxyl groups allowed by the Regulation (EC) No 2003/2003. Since para, meta and ortho methyl positional isomers of the EDDHMA present quite similar stability, they could be grouped: in the method here described the para, meta and ortho methyl positional isomers of the [o,o] EDDHMA are considered together. HBED (N,N’-bis(2-hydroxybenzyl)-ethylenediamine-N,N’-diacetic acid) does not present isomeric forms. NOTE 2 At present, analytically pure standards only exist for [ortho,ortho] EDDHA, [ortho,ortho] EDDHMA and HBED. All other substances being unavailable as a standard, the influence of their eventual presence in the samples (with respect to the sensitivity and the selectivity of this method) has not been studied. NOTE 3 The meso and the dl-racemic forms of [o,o] EDDHA and [o,o] EDDHMA can be determined separately by this method.
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 35 | undefined |
| 41 | 1 Scope 2 Normative references 3 Terms and definitions |
| 42 | 4 Principle 5 Interferences 6 Reagents |
| 44 | 7 Apparatus 8 Sampling and sample preparation |
| 45 | 9 Procedure 9.1 Preparation of the sample solution for iron chelates 9.2 Preparation of the sample solution for other micronutrient chelates 9.3 Preparation of the calibration solutions |
| 46 | 9.4 Chromatographic analysis |
| 49 | 10 Expression of the results 10.1 Fe in Fe-chelates 10.1.1 Fe chelated by [o,o] EDDHA or [o,o] EDDHMA in Fe-[o,o] EDDHA or Fe-[o,o] EDDHMA chelates |
| 50 | 10.1.2 Fe chelated by HBED in Fe-HBED chelates 10.2 Chelating agent in fertilizers 10.2.1 Mass fraction of the chelating agent in fertilizers containing [o,o] EDDHA or [o,o] EDDHMA |
| 51 | 10.2.2 Mass fraction of the chelating agent in fertilizers containing HBED 11 Precision 11.1 Inter-laboratory test 11.2 Repeatability 11.3 Reproducibility |
| 53 | 12 Test report |
| 54 | Annex A (informative)Statistical results of the inter-laboratory tests A.1 Statistical results of the inter-laboratory test performed in 2003 A.1.1 Test samples A.1.2 Inter-laboratory test procedure A.1.3 Results and statistical interpretation |
| 56 | A.2 Statistical results of the inter-laboratory test performed in 2010 A.2.1 Test samples A.2.2 Inter-laboratory test procedure A.2.3 Results and statistical interpretation |
| 57 | A.3 Statistical results of the inter-laboratory test performed in 2014 — Part A A.3.1 Objective A.3.2 Test samples A.3.3 Inter-laboratory test procedure A.3.4 Results and statistical interpretation |
| 59 | A.4 Statistical results of the inter-laboratory test performed in 2014 — Part B A.4.1 Objective A.4.2 Test samples |
| 60 | A.4.3 Inter-laboratory test procedure A.4.4 Results and statistical interpretation |
| 61 | Annex B (informative)General procedure for the determination of the titrimetric purity of the chelating agents using a photometric automatic titrator |
| 62 | Annex C (informative)Complete names of chelating agents |
