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BS EN 17899:2024

BS EN 17899:2024

$142.49

Water quality. Spectrophotometric determination of chlorophyll-a content by ethanol extraction for the routine monitoring of water quality

Published By Publication Date Number of Pages
BSI 2024 24
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This document describes a spectrophotometric method for determining the chlorophyll-a content corrected for phaeopigments as a measure of the amount of phytoplankton for all types of surface water including marine water. The determination limit is usually 2 Āµg/l to 5 Āµg/l and is calculated by each laboratory individually. It can be as low as 0,5 Āµg/l using 2 l of sample (or even more) and a 50 mm cuvette. NOTE In some measurement programs like marine studies on time series data and ecological status/classification no correction for phaeopigments is used and acidification is omitted, e.g. as recommended by OSPAR.

PDF Catalog

PDF Pages PDF Title
2 undefined
8 1 Scope
2 Normative references
3 Terms and definitions
9 4 Principle
5 Interferences
6 Reagents
10 7 Apparatus
11 8 Procedure
8.1 General
8.2 Sample pre-treatment
8.3 Filtration
8.4 Extraction
12 8.5 Measurement
9 Quality assurance
9.1 Blank values
9.2 pH after acidification
9.3 Wavelength setting
13 9.4 Ratio A/A’
10 Calculations
14 11 Expression of results
12 Test report
15 Annex A (informative)Performance data
A.1 Interlaboratory trial study in the Netherlands:
A.2 Validation interlaboratory trial in Germany:
18 Annex B (informative)Theoretical backgrounds
B.1 Meaning of the determination of the chlorophyll-a content
B.2 Acidifying extracts
B.3 Calculation of Phaeopigment concentration
19 Annex C (informative)Control samples
C.1 Introduction
C.2 Surface water
C.3 Frozen spinach extracts
C.4 Freeze-dried kale
20 C.5 Algae culture
21 Annex D (informative)SCOR-Unesco method for determination of chlorophyll in sea-water
D.1 Measurement of chlorophyll a, b, c and d with SCOR-Unesco method using chromatic equations
22 D.2 Performance characteristics and comparison of methods
D.2.1 Validation study in France

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BS EN 17899:2024
$142.49