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BS EN ISO 13885-3:2021

BS EN ISO 13885-3:2021

$142.49

Gel permeation chromatography (GPC) – Water as eluent

Published By Publication Date Number of Pages
BSI 2021 32
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This document specifies the determination of the molar-mass distribution and the average molar mass values Mn (number average) and Mw (weight average) of polymers that are soluble in water by gel permeation chromatography (GPC).

NOTE Also known as size exclusion chromatography (SEC).

This method is applicable to neutral polymers and polyanions (e.g. polycarboxylates, polysaccharides, fully hydrolyzed polyvinyl alcohols and high-molecular polyethylene oxides). It is not applicable to polycations [e.g. polyvinylpyrrolidone, polyvinylpyridine, salts of poly(diallyl‑N,N‑dimethyl‑azacyclopentane), chitosan].

Despite good solubility in the mobile phase and even though the chromatograms obtained show good repeatability, it is possible that this method cannot be used with certain polymer types because of specific interactions (e.g. adsorption) within the sample/eluent/column system (see also Clause 12).

The conditions specified in this document are not applicable to the GPC analysis of polymer samples with Mw values greater than 106 g/mol and/or polymers with elution limits outside the calibration range (see 7.6 and Annex C).

This document includes no correction methods (e.g. for the elimination of peak broadening). If absolute molar mass values are required, an absolute method (e.g. membrane osmometry for Mn or light scattering for Mw) can be used.

PDF Catalog

PDF Pages PDF Title
2 National foreword
4 European foreword
7 Foreword
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Principle
5 Apparatus
11 5.1 Eluent supply
5.2 Pump
5.3 Injection system
5.4 Separation columns
13 5.5 Column temperature control
14 5.6 Detector
6 Reagents
7 Calibration of the apparatus
7.1 General
7.2 Specification for the calibration standard
15 7.3 Preparation of the calibration solutions for injection
7.4 Conditions for calibration runs
7.5 Measurement of elution volume
7.6 Plotting the calibration curve
16 8 Sampling
9 Preparation for the test
9.1 Preparation of the injection solution
17 9.2 Preparation of the apparatus
10 Analytical parameters
11 Data acquisition and evaluation
11.1 General
18 11.2 Calculation of the net chromatogram from the raw data
11.2.1 Determination of the baseline
11.2.2 Correction of the measured values and of the net chromatogram
11.2.3 Evaluation limits
11.3 Calculation of the average values
19 11.4 Calculation of the distribution curves
20 12 Precision
12.1 General
12.2 Repeatability
12.3 Reproducibility
21 13 Test report
13.1 General
13.2 General data on the equipment and settings
13.2.1 Data on the equipment used
13.2.2 Calibration
22 13.2.3 Evaluation
13.3 Special data on the sample
23 Annex A (informative) Conversion of experimental parameters for variant column sizes
24 Annex B (informative) Example of a data sheet for a polymer standard
26 Annex C (informative) Explanations
31 Bibliography

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BS EN ISO 13885-3:2021
$142.49