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BS EN ISO 21572:2019

BS EN ISO 21572:2019

$167.15

Foodstuffs. Molecular biomarker analysis. Immunochemical methods for the detection and quantification of proteins

Published By Publication Date Number of Pages
BSI 2019 36
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This document specifies performance criteria for immunochemical methods for the detection and/or quantification of a specific protein or protein(s) of interest [POI(s)] in a specified matrix.

The methods discussed are applicable to the analysis of proteins from a variety of sample types. Some uses for these methods include, but are not limited to, analysing proteins involved in crop and food production, food processing, food marketing, food safety, biotechnology or disease indexing.

PDF Catalog

PDF Pages PDF Title
2 undefined
4 European foreword
Endorsement notice
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
4 Principle
10 5 Reagents
6 Laboratory equipment
7 Sampling
8 Procedure
8.1 General
8.2 Preparation of sample solution
11 8.3 Extraction
8.4 Preparation of calibration curves, positive controls, and reference materials
8.5 Assay procedure
9 Interpretation and expression of results
9.1 General
12 9.2 Quantitative and semi-quantitative analysis
9.3 Qualitative analysis
10 Specific parameters that can influence results
10.1 General
13 10.2 Special considerations
10.2.1 Selectivity
10.2.2 Extraction efficiency
10.2.3 Matrix effects
10.2.4 Assay applicability
10.2.5 Hook effect
10.2.6 Parallelism/linearity
14 10.2.7 Limits of detection
10.2.8 Limits of quantification
11 Confirming method
12 Test report
16 Annex A (informative) Detection of a protein by ELISA
27 Annex B (informative) Detection of a protein or proteins by lateral flow devices
34 Bibliography

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BS EN ISO 21572:2019
$167.15