BS EN ISO 6888-1:1999+A2:2018

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Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) – Technique using Baird-Parker agar medium

Published By	Publication Date	Number of Pages
BSI	2018	28

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Categories: 07.100.30 - Food microbiology, BSI

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PDF Pages

PDF Title

National foreword

European foreword
Anchor 4
Foreword to amendment A1
Anchor 2
Foreword to amendment A2

Foreword

Introduction

1 Scope
2 Normative references
3 Terms and definitions

4 Principle
5 Diluent and culture media
5.1 General
5.2 Diluent
5.3 Baird-Parker agar medium
5.3.1 Base medium

5.3.2 Solutions

5.3.3 Complete medium
5.3.4 Preparation of agar plates
5.4 Brain-heart infusion broth
5.4.1 Composition

5.4.2 Preparation
5.5 Rabbit plasma
5.6 Rabbit plasma fibrinogen agar (RPFA) medium
5.6.1 General
5.6.2 Base medium
5.6.3 Solutions

5.6.4 Complete medium

5.6.5 Preparation of RPFA plates
6 Apparatus and glassware

7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Test portion, initial suspension and dilutions
9.2 Inoculation

9.3 Incubation
9.4 Selection of plates and interpretation

9.5 Confirmation
9.5.1 General
9.5.2 Coagulase tube test
9.5.3 Plate test using RPFA
10 Expression of results
10.1 General case
10.1.1 Calculation of the number a of coagulase-positive staphylococci identified for each plate selected

10.1.2 Calculation of the number N of identified coagulase-positive staphylococci present in the test portion
10.1.3 Example

10.2 Estimation of low numbers
11 Precision
11.1 General

11.2 Repeatability
11.2.1 Repeatability limit
11.2.2 Overall values
11.3 Reproducibility
11.3.1 Reproducibility limit

12 Test report

Annex A (informative) Results of the interlaboratory study

Bibliography

Pages	28
Descriptors	Equations, Food technology, Animal feed, Test equipment, Biological analysis and testing, Bacteria count methods, Bibliography, Microbiological analysis, Agar, Horizontal, Food products, Specimen preparation, Precision, Cultures (biology), Confidence limits, Coagulation, Culture techniques, Aerobic bacteria, Temperature, Staphylococcus, Test specimens, Count methods (microbiology)
Identical National Standard Of	ISO 6888-1:1999/Amd 2:2018, EN ISO 6888-1:1999/A2:2018
Standard Number	BS EN ISO 6888-1:1999+A2:2018
ISBN	978 0 539 02579 8
Replaced By	BS EN ISO 6888-1:2021, BS EN ISO 6888-1:2021+A1:2023
Withdrawn Date	2021-09-09
Publication Date	2018-10-05
Committee	AW/9
Publisher	BSI
Replaces	BS 5763-7:1983, BS 4285-3.10.1:1989
Corrects	BS EN ISO 6888-1:1999+A2:2018
Title	Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) – Technique using Baird-Parker agar medium
Status	Withdrawn
ICS Codes	07.100.30 - Food microbiology


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BS EN ISO 6888-1:1999+A2:2018

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