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BSI 23/30454764 DC 2023

BSI 23/30454764 DC 2023

$13.70

BS EN 14349. Chemical disinfectants and antiseptics. Quantitative surface test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in the veterinary area on non-porous surfaces without mechanical action. Test method and requirements (phase 2, step 2)

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BSI 2023 39
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This European Standard specifies a test method and the minimum requirements for bactericidal activity of chemical disinfectant and antiseptic products that form a homogeneous physically stable preparation when diluted with hard water, or – in the case of ready-to-use-products – with water. This European Standard applies to products that are used in the veterinary area on non-porous surfaces without mechanical action i.e. in the breeding, husbandry, production, transport and disposal of all animals except when in the food chain following death and entry to the processing industry. EN 14885 specifies in detail the relationship of the various tests to one another and to “use recommendations”. NOTE 1 The method described is intended to determine the activity of commercial formulations or active substances under the conditions in which they are used. NOTE 2 This method corresponds to a Phase 2 Step 2 test. NOTE 3 This method cannot be used to evaluate the activity of products against mycobacteria.

PDF Catalog

PDF Pages PDF Title
7 1 Scope
2 Normative references
3 Terms, definitions and abbreviations
3.1 Terms and definitions
3.2 Symbols and abbreviations
8 4 Requirements
9 5 Test method
5.1 Principle
5.2 Materials and reagents
5.2.1 Test organisms
5.2.2 Culture media and reagents
5.2.2.1 General
10 5.2.2.2 Water
5.2.2.3 Tryptone Soya Agar (TSA)
5.2.2.4 Diluent
5.2.2.5 Neutralizer
11 5.2.2.6 Hard water for dilution of products
5.2.2.7 Interfering substances
12 5.2.3 Test surface
5.3 Apparatus and glassware
5.3.1 General
5.3.2 Usual microbiological laboratory equipment)
13 5.4 Preparation of test organism suspensions and product test solutions
5.4.1 Test organism suspensions (test and validation suspension)
5.4.1.1 General
5.4.1.2 Stock cultures of test organisms
5.4.1.3 Working culture of test organisms
14 5.4.1.4 Test suspension (“N”)
5.4.1.5 Incubation and counting of the test and the validation suspensions
15 5.4.2 Product test solutions
5.5 Procedure for assessing the bactericidal activity of the product
5.5.1 General
5.5.1.1 Experimental conditions
16 5.5.1.2 Neutralization
5.5.1.3 General instruction for validation and control procedures
5.5.1.4 Equilibration of temperature
5.5.2 Test procedure (Dilution-neutralization method))
5.5.2.1 General
5.5.2.2 Test “Na” – Determination of bactericidal concentrations
19 5.5.2.3 Water control “Nw”
20 5.5.2.4 Neutralizer control “B” – Verification of the absence of toxicity of the neutralizer
5.5.2.5 Method validation “C” – Dilution-neutralization validation
21 5.5.2.6 Incubation and counting of the test mixture and the control and validation mixtures
5.5.3 Observation of test surface agar
5.6 Experimental data and calculation
5.6.1 Explanation of terms and abbreviations
5.6.1.1 Overview of the different suspensions and test mixtures
22 5.6.1.2 VC values
5.6.2 Calculation
5.6.2.1 General
5.6.2.2 Determination of VC values
5.6.2.3 Calculation of N
23 5.6.2.4 Calculation of Na and Nw
24 5.6.2.5 Calculation of B and C
25 5.7 Verification of methodology
5.7.1 General
5.7.2 Control of weighted mean counts
5.7.3 Basic limits
5.8 Expression of results and precision
5.8.1 Reduction
26 5.8.2 Control of active and non-active product test solution (5.4.2)
5.8.3 Limiting test organism and bactericidal concentration
5.8.4 Precision, repetitions
5.9 Interpretation of results – conclusion
5.9.1 General
5.9.2 Bactericidal activity for general purposes
5.9.3 Qualification for certain fields of application
27 5.10 Test report
29 Annex A (informative)Referenced strains in national collections
30 Annex B (informative)Examples of neutralizers of the residual antimicrobial activity of chemical disinfectants and antiseptics
32 Annex C (informative)Graphical representation of the method
36 Annex D (informative)Example of a typical test report

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BSI 23/30454764 DC 2023
$13.70