This document specifies one of the methods for evaluating the habitat function of soils and determining the effects of soil contaminants and chemicals on the reproduction of Eisenia fetida\/Eisenia andrei by dermal and alimentary uptake. This chronic test is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, agricultural or other sites concerned, and waste materials. This method is designed mainly for determining the effects of soil contaminants and chemicals on the reproduction of Eisenia fetida\/Eisenia andrei. Technical information is also provided on how to use Eisenia fetida\/andrei for testing chemicals under tropical conditions (see Annex A). Finally, this method also includes technical information on how to use it with other environmentally relevant earthworm species: e.g. Dendrodrilus rubidus and Aporrectodea caliginosa (see Annexes B and C). This method does not apply to substances for which the air\/soil partition coefficient is greater than one, or to substances with vapour pressure exceeding 300 Pa, at 25 \u00b0C. This method does not take into account the persistence of the substance during the test.<\/p>\n
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| 2<\/td>\n | undefined <\/td>\n<\/tr>\n | ||||||
| 4<\/td>\n | European foreword Endorsement notice <\/td>\n<\/tr>\n | ||||||
| 6<\/td>\n | Foreword <\/td>\n<\/tr>\n | ||||||
| 7<\/td>\n | Introduction <\/td>\n<\/tr>\n | ||||||
| 9<\/td>\n | 1 Scope 2 Normative references <\/td>\n<\/tr>\n | ||||||
| 10<\/td>\n | 3 Terms and definitions <\/td>\n<\/tr>\n | ||||||
| 11<\/td>\n | 4 Principle <\/td>\n<\/tr>\n | ||||||
| 12<\/td>\n | 5 Reagents and material <\/td>\n<\/tr>\n | ||||||
| 14<\/td>\n | 6 Apparatus 7 Procedure 7.1 Experimental design 7.1.1 General <\/td>\n<\/tr>\n | ||||||
| 15<\/td>\n | 7.1.2 Preliminary test 7.1.3 Definitive test 7.2 Preparation of test mixtures 7.2.1 Testing of contaminated soil <\/td>\n<\/tr>\n | ||||||
| 16<\/td>\n | 7.2.2 Testing substances added to the control soil 7.2.3 Preparation of control container <\/td>\n<\/tr>\n | ||||||
| 17<\/td>\n | 7.3 Addition of the earthworms 7.4 Test conditions and measurements 7.5 Reference substance <\/td>\n<\/tr>\n | ||||||
| 18<\/td>\n | 8 Calculation and expression of results 8.1 Calculation 8.2 Expression of results 9 Validity of the test 10 Statistical analysis 10.1 General 10.2 Single-concentration tests <\/td>\n<\/tr>\n | ||||||
| 19<\/td>\n | 10.3 Multi-concentration tests 10.3.1 Preliminary test 10.3.2 Definitive test <\/td>\n<\/tr>\n | ||||||
| 20<\/td>\n | 11 Test report <\/td>\n<\/tr>\n | ||||||
| 21<\/td>\n | Annex A (informative) Determination of the chronic toxicity of chemicals on Eisenia fetida\/Eisenia andrei under tropical conditions <\/td>\n<\/tr>\n | ||||||
| 23<\/td>\n | Annex B (informative) Culturing and testing procedures for assessing the effects of contaminants on juvenile production using the earthworm, Dendrodilus rubidus <\/td>\n<\/tr>\n | ||||||
| 27<\/td>\n | Annex C (informative) Culturing and testing procedures for assessing the effects of contaminants on adult survival and cocoon production and hatchability using the earthworm, Aporrectodea caliginosa s.s. <\/td>\n<\/tr>\n | ||||||
| 31<\/td>\n | Annex D (informative) Culturing of Eisenia fetida and Eisenia andrei <\/td>\n<\/tr>\n | ||||||
| 32<\/td>\n | Annex E (normative) Determination of water holding capacity <\/td>\n<\/tr>\n | ||||||
| 33<\/td>\n | Annex F (informative) Techniques for counting juvenile worms hatched from cocoons <\/td>\n<\/tr>\n | ||||||
| 34<\/td>\n | Annex G (informative) Experience with performance criteria of the test <\/td>\n<\/tr>\n | ||||||
| 41<\/td>\n | Bibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" Soil quality. Effects of pollutants on earthworms – Determination of effects on reproduction of Eisenia fetida\/Eisenia andrei and other earthworm species<\/b><\/p>\n |