{"id":430983,"date":"2024-10-20T07:25:45","date_gmt":"2024-10-20T07:25:45","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-15634-32023\/"},"modified":"2024-10-26T14:07:17","modified_gmt":"2024-10-26T14:07:17","slug":"bs-en-15634-32023","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-15634-32023\/","title":{"rendered":"BS EN 15634-3:2023"},"content":{"rendered":"

This document specifies a method for the detection of hazelnut (Corylus avellana) in chocolate. Real-time PCR (Polymerase chain reaction) detection of hazelnut is based on an152 bp (base pair) sequence from the corA 1 gene of hazelnut.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nundefined <\/td>\n<\/tr>\n
7<\/td>\n1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
5.1 General
5.2 Extraction reagents <\/td>\n<\/tr>\n
8<\/td>\n5.3 DNA purification by means of solid phase extraction
5.4 Real-time PCR reagents <\/td>\n<\/tr>\n
9<\/td>\n6 Apparatus and equipment
6.1 General
6.2 DNA extraction
6.3 PCR <\/td>\n<\/tr>\n
10<\/td>\n7 Procedure
7.1 General
7.2 Sample preparation
7.3 Preparation of extracts
7.3.1 DNA extraction with CTAB and DNA purification <\/td>\n<\/tr>\n
11<\/td>\n7.3.2 Quantification and normalization of DNA concentration
7.4 Real-time PCR set-up
7.4.1 Reaction mix for real-time PCR <\/td>\n<\/tr>\n
12<\/td>\n7.4.2 Positive control for DNA targets <\/td>\n<\/tr>\n
13<\/td>\n7.4.3 Negative control for DNA targets
7.4.4 PCR inhibition control
7.4.5 Amplification reagent control
7.4.6 Extraction blank control
7.4.7 Positive extraction control
7.4.8 Temperature\/time programme (real-time PCR) <\/td>\n<\/tr>\n
14<\/td>\n7.4.9 Accept\/Reject criteria
7.4.10 Identification
8 Validation
8.1 General
8.2 Specificity <\/td>\n<\/tr>\n
15<\/td>\n8.3 Ring trial validation study
8.3.1 Setup of the ring trial study
8.3.2 Ring trial validation results
8.3.2.1 Ring trial validation data <\/td>\n<\/tr>\n
16<\/td>\n8.3.2.2 Robustness of the method <\/td>\n<\/tr>\n
17<\/td>\n8.3.2.3 Limit of detection
9 Test report <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Foodstuffs. Detection of food allergens by molecular biological methods – Hazelnut (Corylus avellana). Qualitative detection of a specific DNA sequence in chocolate by real-time PCR<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2023<\/td>\n20<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":430992,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-430983","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/430983","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/430992"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=430983"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=430983"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=430983"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}